Cell Biology Antibodies 1

Anti-COPB1 Antibody (CAB10485)

(No reviews yet) Write a Review
SKU:
CAB10485
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-COPB1 Antibody
Antibody SKU:CAB10485
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 460-740 of human COPB1 (NP_057535.1).
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Raji, LO2, A-549, NIH/3T3, Mouse heart, Mouse kidney, Mouse liver, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 460-740 of human COPB1 (NP_057535.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:WILG EYCS TKED IQSV MTEI RRSL GEIP IVES EIKK EAGE LKPE EEIT VGPV QKLV TEMG TYAT QSAL SSSR PTKK EEDR PPLR GFLL DGDF FVAA SLAT TLTK IALR YVAL VQEK KKQN SFVA EAML LMAT ILHL GKSS LPKK PITD DDVD RISL CLKV LSEC SPLM NDIF NKEC RQSL SHML SAKL EEEK LSQK KESE KRNV TVQP DDPI SFMQ LTAK NEMN CKED QFQL SLLA AMGN TQRK EAAD PLAS KLNK VTQL TGFS DPVY AEAY VHVN QYDI V
Gene ID:1315
Uniprot:P53618
Cellular Location:COPI-coated vesicle membrane, Cell membrane, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Endoplasmic reticulum-Golgi intermediate compartment, Golgi apparatus membrane, Peripheral membrane protein
Calculated MW:107kDa
Observed MW:110kDa
Synonyms:COPB1, COPB
Background:This gene encodes a protein subunit of the coatomer complex associated with non-clathrin coated vesicles. The coatomer complex, also known as the coat protein complex 1, forms in the cytoplasm and is recruited to the Golgi by activated guanosine triphosphatases. Once at the Golgi membrane, the coatomer complex may assist in the movement of protein and lipid components back to the endoplasmic reticulum. Alternatively spliced transcript variants have been described.
UniProt Protein Function:COPB1: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Plays a functional role in facilitating the transport of kappa- type opioid receptor mRNAs into axons and enhances translation of these proteins. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte surface triglyceride lipase (PNPLA2) with the lipid droplet to mediate lipolysis. Involved in the Golgi disassembly and reassembly processes during cell cycle. Involved in autophagy by playing a role in early endosome function. Plays a role in organellar compartmentalization of secretory compartments including endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC), Golgi, trans-Golgi network (TGN) and recycling endosomes, and in biosynthetic transport of CAV1. Promotes degradation of Nef cellular targets CD4 and MHC class I antigens by facilitating their trafficking to degradative compartments.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Vesicle

Chromosomal Location of Human Ortholog: 11p15.2

Cellular Component: Golgi membrane; Golgi apparatus; intracellular membrane-bound organelle; membrane; cytoplasm; plasma membrane; ER-Golgi intermediate compartment; Golgi-associated vesicle; COPI vesicle coat; cytosol

Molecular Function:protein binding; structural molecule activity

Biological Process: intracellular protein transport; intra-Golgi vesicle-mediated transport; COPI coating of Golgi vesicle; viral reproduction; retrograde vesicle-mediated transport, Golgi to ER

NCBI Summary:This gene encodes a protein subunit of the coatomer complex associated with non-clathrin coated vesicles. The coatomer complex, also known as the coat protein complex 1, forms in the cytoplasm and is recruited to the Golgi by activated guanosine triphosphatases. Once at the Golgi membrane, the coatomer complex may assist in the movement of protein and lipid components back to the endoplasmic reticulum. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]
UniProt Code:P53618
NCBI GenInfo Identifier:116241311
NCBI Gene ID:1315
NCBI Accession:P53618.3
UniProt Secondary Accession:P53618,Q6GTT7, Q9NTK2, Q9UNW7, D3DQX0,
UniProt Related Accession:P53618
Molecular Weight:107,142 Da
NCBI Full Name:Coatomer subunit beta
NCBI Synonym Full Names:coatomer protein complex, subunit beta 1
NCBI Official Symbol:COPB1  
NCBI Official Synonym Symbols:COPB  
NCBI Protein Information:coatomer subunit beta; beta coat protein; beta-coat protein; beta-cop
UniProt Protein Name:Coatomer subunit beta
UniProt Synonym Protein Names:Beta-coat protein; Beta-COP
UniProt Gene Name:COPB1  
UniProt Entry Name:COPB_HUMAN
Anti-COPB1 Antibody (CAB10485)Western blot analysis of extracts of various cell lines, using COPB1 antibody (CAB10485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
View AllClose

0 Reviews

View AllClose

Related Products