KO Validated Antibodies 1

Anti-COMT Antibody (CAB18085)[KO Validated]

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SKU:
CAB18085
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism

Description

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Antibody Name:Anti-COMT Antibody [KO Validated]
Antibody SKU:CAB18085
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 42-221 of human COMT (NP_009294.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 42-221 of human COMT (NP_009294.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VGDK KGKI VDAV IQEH QPSV LLEL GAYC GYSA VRMA RLLS PGAR LITI EINP DCAA ITQR MVDF AGVK DKVT LVVG ASQD IIPQ LKKK YDVD TLDM VFLD HWKD RYLP DTLL LEEC GLLR KGTV LLAD NVIC PGAP DFLA HVRG SSCF ECTH YQSF LEYR EVVD GLEK AIYK GPGS EAGP
Gene ID:1312
Uniprot:P21964
Cellular Location:Cell membrane, Cytoplasm, Extracellular side, Single-pass type II membrane protein
Calculated MW:24kDa/30kDa
Observed MW:24kDa, 28kDa
Anti-COMT Antibody [KO Validated] (CAB18085)Western blot analysis of extracts from normal (control) and COMT knockout (KO) 293T cells, using COMT antibody (CAB18085) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Synonyms:COMT, HEL-S-98n
Background:Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters.
UniProt Protein Function:COMT: Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. Belongs to the mammalian catechol-O-methyltransferase family. 2 isoforms of the human protein are produced by alternative initiation.
UniProt Protein Details:

Protein type:Membrane protein, integral; Methyltransferase; EC 2.1.1.6; Amino Acid Metabolism - tyrosine

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: postsynaptic membrane; mitochondrion; membrane; axon; plasma membrane; dendritic spine; integral to membrane; cytosol

Molecular Function:protein binding; magnesium ion binding; O-methyltransferase activity; catechol O-methyltransferase activity

Biological Process: response to drug; methylation; estrogen metabolic process; cellular response to phosphate starvation; neurotransmitter catabolic process; dopamine catabolic process; negative regulation of smooth muscle cell proliferation; short-term memory; response to pain; response to lipopolysaccharide; female pregnancy; learning; reproductive process in a multicellular organism; response to organic cyclic substance; positive regulation of homocysteine metabolic process; negative regulation of dopamine metabolic process; synaptic transmission; xenobiotic metabolic process; regulation of sensory perception of pain; developmental process; neurotransmitter biosynthetic process

Disease: Schizophrenia; Panic Disorder 1

NCBI Summary:Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq, Sep 2008]
UniProt Code:P21964
NCBI GenInfo Identifier:116907
NCBI Gene ID:1312
NCBI Accession:P21964.2
UniProt Secondary Accession:P21964,Q6IB07, Q6ICE6, Q9BWC7, A8MPV9,
UniProt Related Accession:P21964
Molecular Weight:271
NCBI Full Name:Catechol O-methyltransferase
NCBI Synonym Full Names:catechol-O-methyltransferase
NCBI Official Symbol:COMT  
NCBI Official Synonym Symbols:HEL-S-98n  
NCBI Protein Information:catechol O-methyltransferase; epididymis secretory sperm binding protein Li 98n
UniProt Protein Name:Catechol O-methyltransferase
Protein Family:Catechol O-methyltransferase
UniProt Gene Name:COMT  
UniProt Entry Name:COMT_HUMAN
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