Neuroscience

Anti-COLQ Antibody (CAB10448)

(No reviews yet) Write a Review
SKU:
CAB10448
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Neuroscience

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-COLQ Antibody
Antibody SKU:CAB10448
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 276-455 of human COLQ (NP_005668.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse skeletal muscle, Mouse lung, Rat skeletal muscle, Rat heart, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 276-455 of human COLQ (NP_005668.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGPK GERG FPGP PGRC LCGP TMNV NNPS YGES VYGP SSPR VPVI FVVN NQEE LERL NTQN AIAF RRDQ RSLY FKDS LGWL PIQL TPFY PVDY TADQ HGTC GDGL LQPG EECD DGNS DVGD DCIR CHRA YCGD GHRH EGVE DCDG SDFG YLTC ETYL PGSY GDLQ CTQY CYID STPC RYFT
Gene ID:8292
Uniprot:Q9Y215
Cellular Location:Cell junction, synapse
Calculated MW:28kDa/30kDa/33kDa/44kDa/46kDa/47kDa
Observed MW:48kDa
Synonyms:COLQ, CMS5, EAD
Background:This gene encodes the subunit of a collagen-like molecule associated with acetylcholinesterase in skeletal muscle. Each molecule is composed of three identical subunits. Each subunit contains a proline-rich attachment domain (PRAD) that binds an acetylcholinesterase tetramer to anchor the catalytic subunit of the enzyme to the basal lamina. Mutations in this gene are associated with endplate acetylcholinesterase deficiency. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:COLQ: Anchors the catalytic subunits of asymmetric AChE to the synaptic basal lamina. Defects in COLQ are the cause of congenital myasthenic syndrome Engel type (CMSE); also known as end-plate acetylcholinesterase deficiency or congenital myasthenic syndrome type IC (CMS-IC). CMSE is a rare autosomal recessive congenital myasthenic syndrome characterized by onset during childhood, generalized weakness, abnormal fatigability on exertion, refrectoriness to acetylcholinesterase drugs, decremental electromyographic response and morphological abnormalities of the neuromuscular junctions. Belongs to the COLQ family. 8 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Extracellular matrix

Chromosomal Location of Human Ortholog: 3p25

Cellular Component: extracellular space; collagen; synapse; basal lamina; cell junction

Molecular Function:protein binding

Biological Process: asymmetric protein localization; acetylcholine catabolic process in synaptic cleft

Disease: Endplate Acetylcholinesterase Deficiency

NCBI Summary:This gene encodes the subunit of a collagen-like molecule associated with acetylcholinesterase in skeletal muscle. Each molecule is composed of three identical subunits. Each subunit contains a proline-rich attachment domain (PRAD) that binds an acetylcholinesterase tetramer to anchor the catalytic subunit of the enzyme to the basal lamina. Mutations in this gene are associated with endplate acetylcholinesterase deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q9Y215
NCBI GenInfo Identifier:18105016
NCBI Gene ID:8292
NCBI Accession:NP_005668.2
UniProt Related Accession:Q9Y215
Molecular Weight:
NCBI Full Name:acetylcholinesterase collagenic tail peptide isoform I
NCBI Synonym Full Names:collagen like tail subunit of asymmetric acetylcholinesterase
NCBI Official Symbol:COLQ  
NCBI Official Synonym Symbols:EAD; CMS5  
NCBI Protein Information:acetylcholinesterase collagenic tail peptide
UniProt Protein Name:Acetylcholinesterase collagenic tail peptide
UniProt Synonym Protein Names:AChE Q subunit; Acetylcholinesterase-associated collagen
UniProt Gene Name:COLQ  
UniProt Entry Name:COLQ_HUMAN
Anti-COLQ Antibody (CAB10448)Western blot analysis of extracts of various cell lines, using COLQ antibody (CAB10448) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
View AllClose

0 Reviews

View AllClose

Related Products