Anti-Collagen I/COL1A2 Antibody (CAB21059)
- SKU:
- CAB21059
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-Collagen I/COL1A2 Antibody (CAB21059) |
| Antibody SKU: | CAB21059 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 500-600 of human Collagen I/COL1A2 (NP_000080.2). |
| Application: | Western blotting |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse bone, Rat skin |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 500-600 of human Collagen I/COL1A2 (NP_000080.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PTGD PGKN GDKG HAGL AGAR GAPG PDGN NGAQ GPPG PQGV QGGK GEQG PPGP PGFQ GLPG PSGP AGEV GKPG ERGL HGEF GLPG PAGP RGER GPPG ESGA A |
| Cellular Location: | Secreted, extracellular matrix, extracellular space |
| Calculated MW: | 129kDa |
| Observed MW: | 129KDa |
| Synonyms: | COL1A2, OI4 |
| Background: | This gene encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity. Three transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. |
 | Western blot analysis of extracts of Rat skin, using Collagen I/COL1A2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s. |
 | Western blot analysis of extracts of Mouse bone, using Collagen I/COL1A2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s. |
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Collagen I/COL1A2 Monoclonal Antibody (CAB22349)
OverviewProduct Name:Collagen I/COL1A2 Monoclonal AntibodyProduct Code:CAB22349Reactivity:Human, MouseApplications:Western blotti
