Cell Biology Antibodies 7

Anti-COLGALT1 Antibody (CAB16571)

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SKU:
CAB16571
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-COLGALT1 Antibody
Antibody SKU:CAB16571
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 30-230 of human COLGALT1 (NP_078932.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, Mouse lung, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 30-230 of human COLGALT1 (NP_078932.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:APPG ADAY FPEE RWSP ESPL QAPR VLIA LLAR NAAH ALPT TLGA LERL RHPR ERTA LWVA TDHN MDNT STVL REWL VAVK SLYH SVEW RPAE EPRS YPDE EGPK HWSD SRYE HVMK LRQA ALKS ARDM WADY ILFV DADN LILN PDTL SLLI AENK TVVA PMLD SRAA YSNF WCGM TSQG YYKR TPAY IPIR KRDR RGCF A
Gene ID:79709
Uniprot:Q8NBJ5
Cellular Location:Endoplasmic reticulum lumen
Calculated MW:71kDa
Observed MW:70kDa
Synonyms:COLGALT1, GLT25D1
Background:The protein encoded by this gene is one of two enzymes that transfers galactose moieties to hydroxylysine residues of collagen and mannose binding lectin. This gene is constitutively expressed and encodes a soluble protein that localizes to the endoplasmic reticulum.
UniProt Protein Function:GLT25D1: Has a beta-galactosyltransferase activity; transfers beta-galactose to hydroxylysine residues of collagen. Belongs to the glycosyltransferase 25 family.
UniProt Protein Details:

Protein type:Transferase; EC 2.4.1.50

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: endoplasmic reticulum lumen; membrane

Molecular Function:procollagen galactosyltransferase activity

Biological Process: extracellular matrix organization and biogenesis; metabolic process

NCBI Summary:The protein encoded by this gene is one of two enzymes that transfers galactose moieties to hydroxylysine residues of collagen and mannose binding lectin. This gene is constitutively expressed and encodes a soluble protein that localizes to the endoplasmic reticulum. [provided by RefSeq, Dec 2015]
UniProt Code:Q8NBJ5
NCBI GenInfo Identifier:31377697
NCBI Gene ID:79709
NCBI Accession:NP_078932.2
UniProt Secondary Accession:Q8NBJ5,Q8NC64,
UniProt Related Accession:Q8NBJ5
Molecular Weight:71,636 Da
NCBI Full Name:procollagen galactosyltransferase 1
NCBI Synonym Full Names:collagen beta(1-O)galactosyltransferase 1
NCBI Official Symbol:COLGALT1  
NCBI Official Synonym Symbols:GLT25D1  
NCBI Protein Information:procollagen galactosyltransferase 1
UniProt Protein Name:Procollagen galactosyltransferase 1
UniProt Synonym Protein Names:Collagen beta(1-O)galactosyltransferase 1; Glycosyltransferase 25 family member 1; Hydroxylysine galactosyltransferase 1
Protein Family:Procollagen galactosyltransferase
UniProt Gene Name:COLGALT1  
UniProt Entry Name:GT251_HUMAN
Anti-COLGALT1 Antibody (CAB16571)Western blot analysis of extracts of various cell lines, using COLGALT1 antibody (CAB16571) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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