Cell Biology Antibodies 12

Anti-COL4A5 Antibody (CAB9809)

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SKU:
CAB9809
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-COL4A5 Antibody
Antibody SKU:CAB9809
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1462-1691 of human COL4A5 (NP_203699.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:22Rv1, NCI-H460, HepG2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1462-1691 of human COL4A5 (NP_203699.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SSVA HGFL ITRH SQTT DAPQ CPQG TLQV YEGF SLLY VQGN KRAH GQDL GTAG SCLR RFST MPFM FCNI NNVC NFAS RNDY SYWL STPE PMPM SMQP LKGQ SIQP FISR CAVC EAPA VVIA VHSQ TIQI PHCP QGWD SLWI GYSF MMHT SAGA EGSG QALA SPGS CLEE FRSA PFIE CHGR GTCN YYAN SYSF WLAT VDVS DMFS KPQS ETLK AGDL RTRI SRCQ VCMK RT
Gene ID:1287
Uniprot:P29400
Cellular Location:Secreted, basement membrane, extracellular matrix, extracellular space
Calculated MW:161kDa
Observed MW:161kDa
Synonyms:COL4A5, ASLN, ATS, CA54
Background:This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Mutations in this gene are associated with X-linked Alport syndrome, also known as hereditary nephritis. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Alternatively spliced transcript variants have been identified for this gene.
UniProt Protein Function:COL4A5: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A5 are the cause of Alport syndrome X- linked (APSX). APSX is characterized by progressive glomerulonephritis, renal failure, sensorineural deafness, specific eye abnormalities (lenticonous and macular flecks), and glomerular basement membrane defects. The disorder shows considerable heterogeneity in that families differ in the age of end-stage renal disease and the occurrence of deafness. Deletions covering the N-terminal regions of COL4A5 and COL4A6, which are localized in a head-to-head manner, are found in the chromosome Xq22.3 centromeric deletion syndrome. This results in a phenotype with features of diffuse leiomyomatosis and Alport syndrome (DL-ATS). Belongs to the type IV collagen family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Extracellular matrix; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xq22

Cellular Component: endoplasmic reticulum lumen; extracellular region

Biological Process: collagen catabolic process; extracellular matrix organization and biogenesis

Disease: Alport Syndrome, X-linked

UniProt Code:P29400
NCBI GenInfo Identifier:461675
NCBI Gene ID:1287
NCBI Accession:P29400.2
UniProt Secondary Accession:P29400,Q16006, Q16126, Q6LD84, Q7Z700, Q9NUB7,
Molecular Weight:161,632 Da
NCBI Full Name:Collagen alpha-5(IV) chain
UniProt Protein Name:Collagen alpha-5(IV) chain
Protein Family:Collagen
UniProt Gene Name:COL4A5  
UniProt Entry Name:CO4A5_HUMAN
Anti-COL4A5 Polyclonal Antibody (CAB9809)Western blot analysis of extracts of various cell lines, using COL4A5 antibody (CAB9809) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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