Cardiovascular Antibodies

Anti-COL4A2 Antibody (CAB7657)

(No reviews yet) Write a Review
SKU:
CAB7657
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cardiovascular

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-COL4A2 Antibody
Antibody SKU:CAB7657
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1486-1712 of human COL4A2 (NP_001837.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:NCI-H460, HeLa, Mouse lung, Mouse ovary, Rat lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1486-1712 of human COL4A2 (NP_001837.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VSIG YLLV KHSQ TDQE PMCP VGMN KLWS GYSL LYFE GQEK AHNQ DLGL AGSC LARF STMP FLYC NPGD VCYY ASRN DKSY WLST TAPL PMMP VAED EIKP YISR CSVC EAPA IAIA VHSQ DVSI PHCP AGWR SLWI GYSF LMHT AAGD EGGG QSLV SPGS CLED FRAT PFIE CNGG RGTC HYYA NKYS FWLT TIPE QSFQ GSPS ADTL KAGL IRTH ISRC QVCM KNL
Gene ID:1284
Uniprot:P08572
Cellular Location:Secreted, basement membrane, extracellular matrix, extracellular space
Calculated MW:167kDa
Observed MW:200kDa
Synonyms:COL4A2, ICH, POREN2
Background:This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. The C-terminal portion of the protein, known as canstatin, is an inhibitor of angiogenesis and tumor growth. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter.
UniProt Protein Function:COL4A2: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A2 are the cause of porencephaly type 2 (POREN2). POREN2 is a neurologic disorder characterized by a fluid-filled cysts or cavities within the cerebral hemispheres. Affected individuals typically have hemiplegia, seizures, and intellectual disability. Porencephaly type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect in the development of the cerebral ventricles. Defects in COL4A2 are a cause of susceptibility to intracerebral hemorrhage (ICH). ICH is a pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Belongs to the type IV collagen family.
UniProt Protein Details:

Protein type:Secreted; Extracellular matrix; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: extracellular matrix; intracellular membrane-bound organelle; endoplasmic reticulum lumen; collagen type IV; extracellular region

Molecular Function:protein binding; extracellular matrix structural constituent

Biological Process: axon guidance; collagen catabolic process; extracellular matrix disassembly; receptor-mediated endocytosis; negative regulation of angiogenesis; extracellular matrix organization and biogenesis; transcription, DNA-dependent; angiogenesis

Disease: Porencephaly 2; Hemorrhage, Intracerebral, Susceptibility To

NCBI Summary:This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. The C-terminal portion of the protein, known as canstatin, is an inhibitor of angiogenesis and tumor growth. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq, Jul 2008]
UniProt Code:P08572
NCBI GenInfo Identifier:116256354
NCBI Gene ID:1284
NCBI Accession:NP_001837.2
UniProt Secondary Accession:P08572,Q14052, Q548C3, Q5VZA9, Q66K23,
UniProt Related Accession:P08572
Molecular Weight:167,553 Da
NCBI Full Name:collagen alpha-2(IV) chain preproprotein
NCBI Synonym Full Names:collagen, type IV, alpha 2
NCBI Official Symbol:COL4A2  
NCBI Official Synonym Symbols:ICH; POREN2  
NCBI Protein Information:collagen alpha-2(IV) chain; collagen alpha-2(IV) chain; canstatin
UniProt Protein Name:Collagen alpha-2(IV) chain
Protein Family:Collagen
UniProt Gene Name:COL4A2  
UniProt Entry Name:CO4A2_HUMAN
Anti-COL4A2 Antibody (CAB7657)Western blot analysis of extracts of various cell lines, using COL4A2 antibody (CAB7657) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
View AllClose

0 Reviews

View AllClose

Related Products