Cardiovascular Antibodies

Anti-COL4A1 Antibody (CAB10710)

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SKU:
CAB10710
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cardiovascular

Description

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Antibody Name:Anti-COL4A1 Antibody
Antibody SKU:CAB10710
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1445-1669 of human COL4A1 (NP_001836.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:U-87 MG
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1445-1669 of human COL4A1 (NP_001836.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GFLV TRHS QTID DPQC PSGT KILY HGYS LLYV QGNE RAHG QDLG TAGS CLRK FSTM PFLF CNIN NVCN FASR NDYS YWLS TPEP MPMS MAPI TGEN IRPF ISRC AVCE APAM VMAV HSQT IQIP PCPS GWSS LWIG YSFV MHTS AGAE GSGQ ALAS PGSC LEEF RSAP FIEC HGRG TCNY YANA YSFW LATI ERSE MFKK PTPS TLKA GELR THVS RCQV CMRR T
Gene ID:1282
Uniprot:P02462
Cellular Location:Secreted, basement membrane, extracellular matrix, extracellular space
Calculated MW:127kDa/160kDa
Observed MW:160kDa
Synonyms:COL4A1, BSVD, RATOR
Background:This gene encodes a type IV collagen alpha protein. Type IV collagen proteins are integral components of basement membranes. This gene shares a bidirectional promoter with a paralogous gene on the opposite strand. The protein consists of an amino-terminal 7S domain, a triple-helix forming collagenous domain, and a carboxy-terminal non-collagenous domain. It functions as part of a heterotrimer and interacts with other extracellular matrix components such as perlecans, proteoglycans, and laminins. In addition, proteolytic cleavage of the non-collagenous carboxy-terminal domain results in a biologically active fragment known as arresten, which has anti-angiogenic and tumor suppressor properties. Mutations in this gene cause porencephaly, cerebrovascular disease, and renal and muscular defects. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
NCBI Summary:This gene encodes a type IV collagen alpha protein. Type IV collagen proteins are integral components of basement membranes. This gene shares a bidirectional promoter with a paralogous gene on the opposite strand. The protein consists of an amino-terminal 7S domain, a triple-helix forming collagenous domain, and a carboxy-terminal non-collagenous domain. It functions as part of a heterotrimer and interacts with other extracellular matrix components such as perlecans, proteoglycans, and laminins. In addition, proteolytic cleavage of the non-collagenous carboxy-terminal domain results in a biologically active fragment known as arresten, which has anti-angiogenic and tumor suppressor properties. Mutations in this gene cause porencephaly, cerebrovascular disease, and renal and muscular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
UniProt Code:P02462
NCBI GenInfo Identifier:125987809
NCBI Gene ID:1282
NCBI Accession:P02462.3
UniProt Secondary Accession:P02462,Q1P9S9, Q5VWF6, Q86X41, Q8NF88, Q9NYC5, A7E2W4 B1AM70,
UniProt Related Accession:P02462
Molecular Weight:127,981 Da
NCBI Full Name:Collagen alpha-1(IV) chain
NCBI Synonym Full Names:collagen type IV alpha 1 chain
NCBI Official Symbol:COL4A1  
NCBI Official Synonym Symbols:BSVD; RATOR  
NCBI Protein Information:collagen alpha-1(IV) chain
UniProt Protein Name:Collagen alpha-1(IV) chain
Protein Family:Collagen
UniProt Gene Name:COL4A1  
UniProt Entry Name:CO4A1_HUMAN
Anti-COL4A1 Antibody (CAB10710)Western blot analysis of extracts of U-87 MG cells, using COL4A1 Antibody (CAB10710) at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.
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