Cell Biology Antibodies 9

Anti-COL3A1 Antibody (CAB3795)

(No reviews yet) Write a Review
SKU:
CAB3795
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-COL3A1 Antibody
Antibody SKU:CAB3795
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1217-1466 of human COL3A1 (NP_000081.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1217-1466 of human COL3A1 (NP_000081.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:APYY GDEP MDFK INTD EIMT SLKS VNGQ IESL ISPD GSRK NPAR NCRD LKFC HPEL KSGE YWVD PNQG CKLD AIKV FCNM ETGE TCIS ANPL NVPR KHWW TDSS AEKK HVWF GESM DGGF QFSY GNPE LPED VLDV QLAF LRLL SSRA SQNI TYHC KNSI AYMD QASG NVKK ALKL MGSN EGEF KAEG NSKF TYTV LEDG CTKH TGEW SKTV FEYR TRKA VRLP IVDI APYD IGGP DQEF GVDV GPVC FL
Gene ID:1281
Uniprot:P02461
Cellular Location:Secreted, extracellular matrix, extracellular space
Calculated MW:111kDa/138kDa
Observed MW:225KDa
Synonyms:COL3A1, EDS4A
Background:This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
UniProt Protein Function:CO3A1: Collagen type III occurs in most soft connective tissues along with type I collagen. Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3); also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity. Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4). EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas. Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA). AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells. Belongs to the fibrillar collagen family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted; Motility/polarity/chemotaxis; Extracellular matrix; Secreted, signal peptide; Cell adhesion

Chromosomal Location of Human Ortholog: 2q31

Cellular Component: extracellular matrix; extracellular space; endoplasmic reticulum lumen; extracellular region; collagen type III

Molecular Function:integrin binding; protein binding; extracellular matrix structural constituent; platelet-derived growth factor binding; metal ion binding; SMAD binding

Biological Process: integrin-mediated signaling pathway; skin development; platelet activation; axon guidance; receptor-mediated endocytosis; extracellular matrix organization and biogenesis; collagen fibril organization; wound healing; heart development; cell-matrix adhesion; negative regulation of immune response; positive regulation of Rho protein signal transduction; collagen catabolic process; extracellular matrix disassembly; response to radiation; gut development; response to mechanical stimulus; response to cytokine stimulus; transforming growth factor beta receptor signaling pathway; fibril organization and biogenesis; cerebral cortex development; peptide cross-linking; skeletal development; aging

Disease: Ehlers-danlos Syndrome, Type Iv, Autosomal Dominant; Ehlers-danlos Syndrome, Type Iii

NCBI Summary:This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]
UniProt Code:P02461
NCBI GenInfo Identifier:124056490
NCBI Gene ID:1281
NCBI Accession:P02461.4
UniProt Secondary Accession:P02461,P78429, Q15112, Q16403, Q53S91, Q541P8, Q6LDB3 Q6LDJ2, Q6LDJ3, Q7KZ56, D2JYH5, D3DPH4,
UniProt Related Accession:P02461
Molecular Weight:
NCBI Full Name:Collagen alpha-1(III) chain
NCBI Synonym Full Names:collagen, type III, alpha 1
NCBI Official Symbol:COL3A1  
NCBI Official Synonym Symbols:EDS4A  
NCBI Protein Information:collagen alpha-1(III) chain; collagen alpha-1(III) chain; collagen, fetal; alpha1 (III) collagen; Ehlers-Danlos syndrome type IV, autosomal dominant
UniProt Protein Name:Collagen alpha-1(III) chain
Protein Family:Collagen
UniProt Gene Name:COL3A1  
UniProt Entry Name:CO3A1_HUMAN
Anti-COL3A1 Antibody (CAB3795)Western blot analysis of extracts of Mouse brain, using COL3A1 Rabbit pAb (CAB3795) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
View AllClose

0 Reviews

View AllClose

Related Products

Mouse COL3A1 Recombinant Protein (RPES0122)

Mouse COL3A1 Recombinant Protein (RPES0122)

system_update_altDatasheetMouse COL3A1 Recombinant Protein Collagen alpha-1(III) chain?Col3a1? is a secreted protein and belongs to the fibrillar collagen family.It contains 1 fibrillar collagen