Antibody Name:	Anti-Coilin Antibody
Antibody SKU:	CAB6428
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 297-576 of human Coilin (NP_004636.1).

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, HepG2, 293T, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 297-576 of human Coilin (NP_004636.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KLGF SLTP SKGK TSGT TSSS SDSS AESD DQCL MSSS TPEC AAGF LKTV GLFA GRGR PGPG LSSQ TAGA AGWR RSGS NGGG QAPG ASPS VSLP ASLG RGWG REEN LFSW KGAK GRGM RGRG RGRG HPVS CVVN RSTD NQRQ QQLN DVVK NSST IIQN PVET PKKD YSLL PLLA AAPQ VGEK IAFK LLEL TSSY SPDV SDYK EGRI LSHN PETQ QVDI EILS SLPA LREP GKFD LVYH NENG AEVV EYAV TQES KITV FWKE LIDP RLII ESPS NTSS TEPA
Gene ID:	8161
Uniprot:	P38432
Cellular Location:	Cajal body, Nucleus
Calculated MW:	62kDa
Observed MW:	80kDa

Synonyms:

COIL, CLN80, p80-coilin, coilin, Coilin

Background:

The protein encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14.

UniProt Protein Function:	coilin: an ubiquitous protein found in nuclear coiled bodies (CBS), nuclear suborganelles involved in the post-transcriptional modification of snRNPs and small nucleolar RNAs. snRNPs undergo 2'-O-methylation and pseudouridylation in CBS. Differential methylation and phosphorylation of coilin likely influences localization among nuclear bodies and the composition and assembly of CBS. Located in the interchromatin space between the nucleolus and the nucleus.
UniProt Protein Details:	Protein type:Oxidoreductase Chromosomal Location of Human Ortholog: 17q22 Cellular Component: nucleoplasm; Cajal body; membrane; cytoplasm; nucleolus; nucleus Molecular Function:protein C-terminus binding; identical protein binding; protein binding
NCBI Summary:	The protein encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14. [provided by RefSeq, Jul 2008]
UniProt Code:	P38432
NCBI GenInfo Identifier:	585632
NCBI Gene ID:	8161
NCBI Accession:	P38432.1
UniProt Secondary Accession:	P38432,B2R931,
UniProt Related Accession:	P38432
Molecular Weight:	576
NCBI Full Name:	Coilin
NCBI Synonym Full Names:	coilin
NCBI Official Symbol:	COIL
NCBI Official Synonym Symbols:	CLN80; p80-coilin
NCBI Protein Information:	coilin; p80; coilin p80
UniProt Protein Name:	Coilin
UniProt Synonym Protein Names:	p80-coilin
Protein Family:	Coilin
UniProt Gene Name:	COIL
UniProt Entry Name:	COIL_HUMAN

	Western blot analysis of extracts of various cell lines, using Coilin antibody (CAB6428) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of HeLa cells using Coilin antibody (CAB6428).