Name: Anti-COG4 Antibody (CAB7792)
Brand: Cell Biology Antibodies 11
SKU: CAB7792
Availability: OutOfStock

Antibody Name:	Anti-COG4 Antibody
Antibody SKU:	CAB7792
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 510-789 of human COG4 (NP_056201.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 510-789 of human COG4 (NP_056201.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FQDI QRGV TSAV NIMH SSLQ QGKF DTKG IEST DEAK MSFL VTLN NVEV CSEN ISTL KKTL ESDC TKLF SQGI GGEQ AQAK FDSC LSDL AAVS NKFR DLLQ EGLT ELNS TAIK PQVQ PWIN SFFS VSHN IEEE EFND YEAN DPWV QQFI LNLE QQMA EFKA SLSP VIYD SLTG LMTS LVAV ELEK VVLK STFN RLGG LQFD KELR SLIA YLTT VTTW TIRD KFAR LSQM ATIL NLER VTEI LDYW GPNS GPLT WRLT PAEV RQVL ALRI DFRS EDIK RLRL
Gene ID:	25839
Uniprot:	Q9H9E3
Cellular Location:	Cytoplasmic side, Golgi apparatus membrane, Peripheral membrane protein
Calculated MW:	38kDa/81kDa/89kDa
Observed MW:	89kDa

Synonyms:	COG4, CDG2J, COD1
Background:	The protein encoded by this gene is a component of an oligomeric protein complex involved in the structure and function of the Golgi apparatus. Defects in this gene may be a cause of congenital disorder of glycosylation type IIj. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	COG4: Required for normal Golgi function. Plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with SCFD1. Defects in COG4 are the cause of congenital disorder of glycosylation type 2J (CDG2J). It is a multisystem disorder caused by a defect in glycoprotein biosynthesis and characterized by under-glycosylated serum glycoproteins. Congenital disorders of glycosylation result in a wide variety of clinical features, such as defects in the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. Belongs to the COG4 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Vesicle; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 16q22.1 Cellular Component: Golgi membrane; Golgi transport complex; trans-Golgi network membrane Molecular Function:protein binding Biological Process: ER to Golgi vesicle-mediated transport; Golgi organization and biogenesis; Golgi vesicle prefusion complex stabilization; retrograde transport, vesicle recycling within Golgi; retrograde vesicle-mediated transport, Golgi to ER Disease: Congenital Disorder Of Glycosylation, Type Iij
NCBI Summary:	The protein encoded by this gene is a component of an oligomeric protein complex involved in the structure and function of the Golgi apparatus. Defects in this gene may be a cause of congenital disorder of glycosylation type IIj. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2010]
UniProt Code:	Q9H9E3
NCBI GenInfo Identifier:	311033464
NCBI Gene ID:	25839
NCBI Accession:	Q9H9E3.3
UniProt Secondary Accession:	Q9H9E3,Q96D40, Q9BRF0, Q9BVZ2, Q9H5Y4, Q9Y3W3, B4DMN8 C9JS23,
UniProt Related Accession:	Q9H9E3
Molecular Weight:	81,098 Da
NCBI Full Name:	Conserved oligomeric Golgi complex subunit 4
NCBI Synonym Full Names:	component of oligomeric golgi complex 4
NCBI Official Symbol:	COG4
NCBI Official Synonym Symbols:	COD1; CDG2J
NCBI Protein Information:	conserved oligomeric Golgi complex subunit 4
UniProt Protein Name:	Conserved oligomeric Golgi complex subunit 4
UniProt Synonym Protein Names:	Component of oligomeric Golgi complex 4
Protein Family:	Conserved oligomeric Golgi complex
UniProt Gene Name:	COG4
UniProt Entry Name:	COG4_HUMAN

	Western blot analysis of extracts of mouse testis, using COG4 antibody (CAB7792) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using COG4 antibody (CAB7792) at dilution of 1:100. Blue: DAPI for nuclear staining.