Cell Biology Antibodies 5
Anti-COA3 Antibody (CAB14585)
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- SKU:
- CAB14585
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-COA3 Antibody |
| Antibody SKU: | CAB14585 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-106 of human COA3 (NP_001035521.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | MCF7 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-106 of human COA3 (NP_001035521.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MASS GAGD PLDS KRGE APFA QRID PTRE KLTP EQLH SMRQ AELA QWQK VLPR RRTR NIVT GLGI GALV LAIY GYTF YSIS QERF LDEL EDEA KAAR ARAL ARAS GS |
| Gene ID: | 28958 |
| Uniprot: | Q9Y2R0 |
| Cellular Location: | Mitochondrion inner membrane, Single-pass membrane protein |
| Calculated MW: | 11kDa |
| Observed MW: | 12kDa |
| Synonyms: | COA3, CCDC56, COX25, HSPC009, MITRAC12 |
| Background: | This gene encodes a member of the cytochrome c oxidase assembly factor family. Studies of a related gene in fly suggest that the encoded protein is localized to mitochondria and is essential for cytochrome c oxidase function. |
| UniProt Protein Function: | CCDC56: Putative COX assembly factor. Belongs to the COA3 family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Mitochondrial Chromosomal Location of Human Ortholog: 17q21 Cellular Component: mitochondrion; integral to mitochondrial inner membrane Molecular Function:protein binding Biological Process: mitochondrial respiratory chain complex IV assembly |
| NCBI Summary: | This gene encodes a member of the cytochrome c oxidase assembly factor family. Studies of a related gene in fly suggest that the encoded protein is localized to mitochondria and is essential for cytochrome c oxidase function. [provided by RefSeq, Nov 2012] |
| UniProt Code: | Q9Y2R0 |
| NCBI GenInfo Identifier: | 94536771 |
| NCBI Gene ID: | 28958 |
| NCBI Accession: | NP_001035521.1 |
| UniProt Secondary Accession: | Q9Y2R0,A8K498, |
| UniProt Related Accession: | Q9Y2R0 |
| Molecular Weight: | 11,731 Da |
| NCBI Full Name: | cytochrome c oxidase assembly factor 3 homolog, mitochondrial |
| NCBI Synonym Full Names: | cytochrome c oxidase assembly factor 3 |
| NCBI Official Symbol: | COA3 |
| NCBI Official Synonym Symbols: | CCDC56; HSPC009; MITRAC12 |
| NCBI Protein Information: | cytochrome c oxidase assembly factor 3 homolog, mitochondrial |
| UniProt Protein Name: | Cytochrome c oxidase assembly factor 3 homolog, mitochondrial |
| UniProt Synonym Protein Names: | Coiled-coil domain-containing protein 56; Mitochondrial translation regulation assembly intermediate of cytochrome c oxidase protein of 12 kDa |
| Protein Family: | Cytochrome c oxidase assembly factor |
| UniProt Gene Name: | COA3 |
| UniProt Entry Name: | COA3_HUMAN |
 | Western blot analysis of extracts of MCF7 cells, using COA3 antibody (CAB14585) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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