Epigenetics & Nuclear Signaling Antibodies 1

Anti-CNOT8 Antibody (CAB12548)

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SKU:
CAB12548
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-CNOT8 Antibody
Antibody SKU:CAB12548
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human CNOT8 (NP_004770.4).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, 293T, Mouse thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human CNOT8 (NP_004770.4).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPAA LVEN SQVI CEVW ASNL EEEM RKIR EIVL SYSY IAMD TEFP GVVV RPIG EFRS SIDY QYQL LRCN VDLL KIIQ LGLT FTNE KGEY PSGI NTWQ FNFK FNLT EDMY SQDS IDLL ANSG LQFQ KHEE EGID TLHF AELL MTSG VVLC DNVK WLSF HSGY DFGY MVKL LTDS RLPE EEHE FFHI LNLF FPSI YDVK YLMK SCKN LKGG LQEV ADQL DLQR IGRQ HQAG SDSL LTGM AFFR MKEL FFED SIDD AKYC GRLY GLGT GVAQ KQNE DVDS AQEK MSIL AIIN NMQQ
Gene ID:9337
Uniprot:Q9UFF9
Cellular Location:Cytoplasm, Nucleus
Calculated MW:21kDa/27kDa/33kDa
Observed MW:34kDa
Synonyms:CNOT8, CAF1, CALIF, Caf1b, POP2, hCAF1
Background:
UniProt Protein Function:CNOT8: Ubiquitous transcription factor required for a diverse set of processes. The CCR4-NOT complex functions as general transcription regulation complex. Belongs to the CAF1 family.
UniProt Protein Details:

Protein type:EC 3.1.13.4

Chromosomal Location of Human Ortholog: 5q31-q33

Cellular Component: intracellular; cytosol; nucleus; CCR4-NOT complex

Molecular Function:protein binding; 3'-5'-exoribonuclease activity; poly(A)-specific ribonuclease activity; RNA binding; metal ion binding; transcription factor activity

Biological Process: regulation of translation; negative regulation of cell proliferation; poly(A) tail shortening; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; gene expression; miRNA-mediated gene silencing; mRNA catabolic process, deadenylation-dependent decay

UniProt Code:Q9UFF9
NCBI GenInfo Identifier:15213949
NCBI Gene ID:9337
NCBI Accession:Q9UFF9.1
UniProt Secondary Accession:Q9UFF9,O95709, Q7Z521, Q9H6Y1, B0AZS3, B2RAR8, B7Z8R1 D3DQI8,
UniProt Related Accession:Q9UFF9
Molecular Weight:27,415 Da
NCBI Full Name:CCR4-NOT transcription complex subunit 8
NCBI Synonym Full Names:CCR4-NOT transcription complex, subunit 8
NCBI Official Symbol:CNOT8  
NCBI Official Synonym Symbols:CAF1; POP2; CALIF; Caf1b; hCAF1  
NCBI Protein Information:CCR4-NOT transcription complex subunit 8; CAF2; CALIFp; CAF1-like protein; CCR4-associated factor 8; PGK promoter directed over production
UniProt Protein Name:CCR4-NOT transcription complex subunit 8
UniProt Synonym Protein Names:CAF1-like protein; CALIFp; CAF2; CCR4-associated factor 8; Caf1b
Protein Family:CCR4-NOT transcription complex
UniProt Gene Name:CNOT8  
UniProt Entry Name:CNOT8_HUMAN
Anti-CNOT8 Antibody (CAB12548)Western blot analysis of extracts of various cell lines, using CNOT8 antibody (CAB12548) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
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