Antibody Name:	Anti-CNOT7 Antibody
Antibody SKU:	CAB8959
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 90-160 of human CNOT7 (NP_037486.2).

Application:	WB IHC
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	22Rv1, U-87MG, HT-29, Raji, HeLa, Mouse lung, Mouse brain, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 90-160 of human CNOT7 (NP_037486.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PGTS TWQF NFKF NLTE DMYA QDSI ELLT TSGI QFKK HEEE GIET QYFA ELLM TSGV VLCE GVKW LSFH SGY
Gene ID:	29883
Uniprot:	Q9UIV1
Cellular Location:	Cytoplasm, Nucleus, P-body
Calculated MW:	28kDa/32kDa
Observed MW:	33kDa

Synonyms:

CNOT7, CAF-1, CAF1, Caf1a, hCAF-1

Background:

The protein encoded by this gene binds to an anti-proliferative protein, B-cell translocation protein 1, which negatively regulates cell proliferation. Binding of the two proteins, which is driven by phosphorylation of the anti-proliferative protein, causes signaling events in cell division that lead to changes in cell proliferation associated with cell-cell contact. The encoded protein downregulates the innate immune response and therefore provides a therapeutic target for enhancing its antimicrobial activity against foreign agents. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and X.

UniProt Protein Function:	CNOT7: Ubiquitous transcription factor required for a diverse set of processes. It is a component of the CCR4 complex involved in the control of gene expression. Belongs to the CAF1 family.
UniProt Protein Details:	Protein type:EC 3.1.13.4; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 8p22-p21.3 Cellular Component: membrane; nucleus; cytosol; CCR4-NOT complex Molecular Function:protein binding; signal transducer activity; 3'-5'-exoribonuclease activity; poly(A)-specific ribonuclease activity; exoribonuclease activity; RNA binding; metal ion binding; transcription factor activity Biological Process: deadenylation-dependent decapping; transcription, DNA-dependent; signal transduction; miRNA-mediated gene silencing; regulation of translation; negative regulation of cell proliferation; poly(A) tail shortening; carbohydrate metabolic process; positive regulation of cell proliferation; gene expression; RNA-mediated gene silencing; positive regulation of transcription from RNA polymerase II promoter; cytoplasmic mRNA processing body assembly; mRNA catabolic process, deadenylation-dependent decay
NCBI Summary:	The protein encoded by this gene binds to an anti-proliferative protein, B-cell translocation protein 1, which negatively regulates cell proliferation. Binding of the two proteins, which is driven by phosphorylation of the anti-proliferative protein, causes signaling events in cell division that lead to changes in cell proliferation associated with cell-cell contact. The encoded protein downregulates the innate immune response and therefore provides a therapeutic target for enhancing its antimicrobial activity against foreign agents. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Sep 2014]
UniProt Code:	Q9UIV1
NCBI GenInfo Identifier:	41713629
NCBI Gene ID:	29883
NCBI Accession:	Q9UIV1.3
UniProt Secondary Accession:	Q9UIV1,Q7Z530, A8MZM5, B3KMP1, B3KN35, D3DSP6, G3V108
UniProt Related Accession:	Q9UIV1
Molecular Weight:	28,228 Da
NCBI Full Name:	CCR4-NOT transcription complex subunit 7
NCBI Synonym Full Names:	CCR4-NOT transcription complex, subunit 7
NCBI Official Symbol:	CNOT7
NCBI Official Synonym Symbols:	CAF1; Caf1a; hCAF-1
NCBI Protein Information:	CCR4-NOT transcription complex subunit 7; CAF-1; BTG1 binding factor 1; BTG1-binding factor 1; CCR4-associated factor 1; carbon catabolite repressor protein (CCR4)-associative factor 1
UniProt Protein Name:	CCR4-NOT transcription complex subunit 7
UniProt Synonym Protein Names:	BTG1-binding factor 1; CCR4-associated factor 1; CAF-1; Caf1a
Protein Family:	CCR4-NOT transcription complex
UniProt Gene Name:	CNOT7
UniProt Entry Name:	CNOT7_HUMAN

	Western blot analysis of extracts of various cell lines, using CNOT7 antibody (CAB8959) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded Mouse brain using CNOT7 Rabbit pAb (CAB8959) at dilution of 1:50 (40x lens).
	Immunohistochemistry of paraffin-embedded Rat brain using CNOT7 Rabbit pAb (CAB8959) at dilution of 1:50 (40x lens).
	Immunohistochemistry of paraffin-embedded Human colon using CNOT7 Rabbit pAb (CAB8959) at dilution of 1:50 (40x lens).