Signal Transduction Antibodies 1

Anti-CNKSR2 Antibody (CAB14578)

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SKU:
CAB14578
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-CNKSR2 Antibody
Antibody SKU:CAB14578
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 400-750 of human CNKSR2 (NP_055742.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:U-87MG, Jurkat, HepG2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 400-750 of human CNKSR2 (NP_055742.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RKRF NIVE EDTV LYCY EYEK GRSS SQGR REST PTYG KLRP ISMP VEYN WVGD YEDP NKMK RDSR RENS LLRY MSNE KIAQ EEYM FQRN SKKD TGKK SKKK GDKS NSPT HYSL LPSL QMDA LRQD IMGT PVPE TTLY HTFQ QSSL QHKS KKKN KGPI AGKS KRRI SCKD LGRG DCEG WLWK KKDA KSYF SQKW KKYW FVLK DASL YWYI NEED EKAE GFIS LPEF KIDR ASEC RKKY AFKA CHPK IKSF YFAA EHLD DMNR WLNR INML TAGY AERE RIKQ EQDY WSES DKEE ADTP STPK QDSP PPPY DTYP RPPS MSCA SPYV EAKH SRLS STET SQSQ SSHE EFRQ EVT
Gene ID:22866
Uniprot:Q8WXI2
Cellular Location:Cytoplasm, Membrane, Peripheral membrane protein
Calculated MW:96kDa/101kDa/113kDa/117kDa
Observed MW:117kDa
Synonyms:CNKSR2, CNK2, KSR2, MAGUIN
Background:This gene encodes a multidomain protein that functions as a scaffold protein to mediate the mitogen-activated protein kinase pathways downstream from Ras. This gene product is induced by vitamin D and inhibits apoptosis in certain cancer cells. It may also play a role in ternary complex assembly of synaptic proteins at the postsynaptic membrane and coupling of signal transduction to membrane/cytoskeletal remodeling. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:CNKSR2: a RAF-binding multidomain protein required for RAS signaling. Contains several protein-protein interaction domains. Its PH domain interacts with Rho in a GTP-dependent manner. Associates with Rhophilin and RalGDS, Rho and Ras effector molecules. Two alternatively spliced isoforms have been described.
UniProt Protein Details:

Protein type:Adaptor/scaffold

Chromosomal Location of Human Ortholog: Xp22.12

Cellular Component: Golgi apparatus; postsynaptic membrane; neuron projection; cell soma; plasma membrane

Molecular Function:identical protein binding

Biological Process: regulation of signal transduction

NCBI Summary:This gene encodes a multidomain protein that functions as a scaffold protein to mediate the mitogen-activated protein kinase pathways downstream from Ras. This gene product is induced by vitamin D and inhibits apoptosis in certain cancer cells. It may also play a role in ternary complex assembly of synaptic proteins at the postsynaptic membrane and coupling of signal transduction to membrane/cytoskeletal remodeling. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]
UniProt Code:Q8WXI2
NCBI GenInfo Identifier:281306725
NCBI Gene ID:22866
NCBI Accession:NP_001162118.1
UniProt Related Accession:Q8WXI2
Molecular Weight:
NCBI Full Name:connector enhancer of kinase suppressor of ras 2 isoform 2
NCBI Synonym Full Names:connector enhancer of kinase suppressor of Ras 2
NCBI Official Symbol:CNKSR2  
NCBI Official Synonym Symbols:CNK2; KSR2; MAGUIN; MRXSHG  
NCBI Protein Information:connector enhancer of kinase suppressor of ras 2
UniProt Protein Name:Connector enhancer of kinase suppressor of ras 2
UniProt Synonym Protein Names:CNK homolog protein 2
UniProt Gene Name:CNKSR2  
UniProt Entry Name:CNKR2_HUMAN
Anti-CNKSR2 Antibody (CAB14578)Western blot analysis of extracts of various cell lines, using CNKSR2 antibody (CAB14578) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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