Signal Transduction Antibodies 2

Anti-CNGA3 Antibody (CAB3288)

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SKU:
CAB3288
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-CNGA3 Antibody
Antibody SKU:CAB3288
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human CNGA3 (NP_001289.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:BT-474, U-87MG, 293T, Mouse pancreas, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human CNGA3 (NP_001289.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAKI NTQY SHPS RTHL KVKT SDRD LNRA ENGL SRAH SSSE ETSS VLQP GIAM ETRG LADS GQGS FTGQ GIAR LSRL IFLL RRWA ARHV HHQD QGPD SFPD RFRG AELK EVSS QESN AQAN VGSQ EPAD RGRS AWPL AKCN TNTS NNTE EEKK TKKK DAIV VDPS S
Gene ID:1261
Uniprot:Q16281
Cellular Location:Membrane, Multi-pass membrane protein
Calculated MW:76-79kDa
Observed MW:110kDa
Synonyms:CNGA3, ACHM2, CCNC1, CCNCa, CCNCalpha, CNCG3, CNG3
Background:This gene encodes a member of the cyclic nucleotide-gated cation channel protein family which is required for normal vision and olfactory signal transduction. Mutations in this gene are associated with achromatopsia (rod monochromacy) and color blindness. Two alternatively spliced transcripts encoding different isoforms have been described.
UniProt Protein Function:CNGA3: Visual signal transduction is mediated by a G-protein coupled cascade using cGMP as second messenger. This protein can be activated by cyclic GMP which leads to an opening of the cation channel and thereby causing a depolarization of cone photoreceptors. Induced a flickering channel gating, weakened the outward rectification in the presence of extracellular calcium, increased sensitivity for L-cis diltiazem and enhanced the cAMP efficacy of the channel when coexpressed with CNGB3. Essential for the generation of light-evoked electrical responses in the red-, green- and blue sensitive cones. Defects in CNGA3 are the cause of achromatopsia type 2 (ACHM2); also known as total colorblindness or rod monochromacy (RMCH2). ACHM2 is an autosomal recessive condition characterized by day blindness and photophobia. In ACHM2 patients the cones are defective and the subjects see better at night. Defects in CNGA3 may be a cause of Leber congenital amaurosis (LCA), a severe dystrophy of the retina, typically becoming evident in the first years of life. Visual function is usually poor and often accompanied by nystagmus, sluggish or near- absent pupillary responses, photophobia, high hyperopia and keratoconus. Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family. CNGA3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Membrane protein, multi-pass; Channel, ligand-gated

Chromosomal Location of Human Ortholog: 2q11.2

Cellular Component: integral to plasma membrane; cytoplasm; dendrite; perikaryon

Molecular Function:protein C-terminus binding; voltage-gated potassium channel activity; intracellular cAMP activated cation channel activity; intracellular cGMP activated cation channel activity; cGMP binding; ligand-gated ion channel activity

Biological Process: phototransduction, visible light; response to magnesium ion; regulation of membrane potential; response to cAMP; visual perception; retinal cone cell development; transport; signal transduction; cation transport; response to corticosteroid stimulus

Disease: Achromatopsia 2

NCBI Summary:This gene encodes a member of the cyclic nucleotide-gated cation channel protein family which is required for normal vision and olfactory signal transduction. Mutations in this gene are associated with achromatopsia (rod monochromacy) and color blindness. Two alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Code:Q16281
NCBI GenInfo Identifier:13959682
NCBI Gene ID:1261
NCBI Accession:Q16281.2
UniProt Secondary Accession:Q16281,Q4VAP7, Q53RD2, Q6ZNA7, Q9UP64, E9PF93,
UniProt Related Accession:Q16281
Molecular Weight:Calculated MW 76-79kDaObserved MW 79kDa
NCBI Full Name:Cyclic nucleotide-gated cation channel alpha-3
NCBI Synonym Full Names:cyclic nucleotide gated channel alpha 3
NCBI Official Symbol:CNGA3  
NCBI Official Synonym Symbols:CNG3; ACHM2; CCNC1; CCNCa; CNCG3; CCNCalpha  
NCBI Protein Information:cyclic nucleotide-gated cation channel alpha-3; CNG-3; CNG channel alpha-3; cyclic nucleotide-gated channel alpha-3; cone photoreceptor cGMP-gated channel alpha subunit; cone photoreceptor cGMP-gated channel subunit alpha
UniProt Protein Name:Cyclic nucleotide-gated cation channel alpha-3
UniProt Synonym Protein Names:Cone photoreceptor cGMP-gated channel subunit alpha; Cyclic nucleotide-gated channel alpha-3; CNG channel alpha-3; CNG-3; CNG3
Protein Family:Cyclic nucleotide-gated cation channel
UniProt Gene Name:CNGA3  
UniProt Entry Name:CNGA3_HUMAN
Anti-CNGA3 Antibody (CAB3288)Western blot analysis of extracts of various cell lines, using CNGA3 antibody (CAB3288) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
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