Metabolism Antibodies 2
Anti-CMPK1 Antibody (CAB6561)
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- SKU:
- CAB6561
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-CMPK1 Antibody |
| Antibody SKU: | CAB6561 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 69-228 of human CMPK1 (NP_057392.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | 293T, HL-60, HT-29, HeLa, Mouse brain, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 69-228 of human CMPK1 (NP_057392.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LLRD ERKN PDSQ YGEL IEKY IKEG KIVP VEIT ISLL KREM DQTM AANA QKNK FLID GFPR NQDN LQGW NKTM DGKA DVSF VLFF DCNN EICI ERCL ERGK SSGR SDDN RESL EKRI QTYL QSTK PIID LYEE MGKV KKID ASKS VDEV FDEV VQIF DKEG |
| Gene ID: | 51727 |
| Uniprot: | P30085 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 16kDa/22kDa |
| Observed MW: | 22kDa |
| Synonyms: | CMPK1, CK, CMK, CMPK, UMK, UMP-CMPK, UMPK |
| Background: | This gene encodes one of the enzymes required for cellular nucleic acid biosynthesis. This enzyme catalyzes the transfer of a phosphate group from ATP to CMP, UMP, or dCMP, to form the corresponding diphosphate nucleotide. Alternate splicing results in both coding and non-coding transcript variants. |
| UniProt Protein Function: | CMPK: Catalyzes specific phosphoryl transfer from ATP to UMP and CMP. Belongs to the adenylate kinase family. |
| UniProt Protein Details: | Protein type:EC 2.7.4.14; Nucleotide Metabolism - pyrimidine; EC 2.7.4.6; Kinase, other Chromosomal Location of Human Ortholog: 1p32 Cellular Component: cytoplasm; cytosol; nucleus Molecular Function:ATP binding; cytidylate kinase activity; nucleoside diphosphate kinase activity; uridine kinase activity; uridylate kinase activity Biological Process: 'de novo' pyrimidine base biosynthetic process; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; nucleoside diphosphate phosphorylation; nucleoside triphosphate biosynthetic process; pyrimidine ribonucleotide biosynthetic process; UMP biosynthetic process |
| NCBI Summary: | This gene encodes one of the enzymes required for cellular nucleic acid biosynthesis. This enzyme catalyzes the transfer of a phosphate group from ATP to CMP, UMP, or dCMP, to form the corresponding diphosphate nucleotide. Alternate splicing results in both coding and non-coding transcript variants. [provided by RefSeq, Feb 2012] |
| UniProt Code: | P30085 |
| NCBI GenInfo Identifier: | 12644008 |
| NCBI Gene ID: | 51727 |
| NCBI Accession: | P30085.3 |
| UniProt Secondary Accession: | P30085,Q53GB7, Q5SVZ0, Q96C07, Q9UBQ8, Q9UIA2, B4DPU7 E9PGI8, |
| UniProt Related Accession: | P30085 |
| Molecular Weight: | 16,548 Da |
| NCBI Full Name: | UMP-CMP kinase |
| NCBI Synonym Full Names: | cytidine/uridine monophosphate kinase 1 |
| NCBI Official Symbol: | CMPK1 |
| NCBI Official Synonym Symbols: | CK; CMK; UMK; CMPK; UMPK; UMP-CMPK |
| NCBI Protein Information: | UMP-CMP kinase |
| UniProt Protein Name: | UMP-CMP kinase |
| UniProt Synonym Protein Names: | Deoxycytidylate kinase |
| UniProt Gene Name: | CMPK1 |
| UniProt Entry Name: | KCY_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CMPK1 antibody (CAB6561) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of MCF7 cells using CMPK1 antibody (CAB6561). Blue: DAPI for nuclear staining. |
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