Name: Anti-CLSTN1 Antibody (CAB15405)
Brand: Cell Biology Antibodies 6
SKU: CAB15405
Availability: OutOfStock

Antibody Name:	Anti-CLSTN1 Antibody
Antibody SKU:	CAB15405
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 29-350 of human CLSTN1 (NP_055759.3).

Application:	WB IF
Recommended Dilution:	WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:	Mouse, Rat
Positive Samples:	Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 29-350 of human CLSTN1 (NP_055759.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ARVN KHKP WLEP TYHG IVTE NDNT VLLD PPLI ALDK DAPL RFAG EICG FKIH GQNV PFDA VVVD KSTG EGVI RSKE KLDC ELQK DYSF TIQA YDCG KGPD GTNV KKSH KATV HIQV NDVN EYAP VFKE KSYK ATVI EGKQ YDSI LRVE AVDA DCSP QFSQ ICSY EIIT PDVP FTVD KDGY IKNT EKLN YGKE HQYK LTVT AYDC GKKR ATED VLVK ISIK PTCT PGWQ GWNN RIEY EPGT GALA VFPN IHLE TCDE PVAS VQAT VELE TSHI GKGC DRDT YSEK SLHR LCGA AAGT AELL PSPS GSLN WTMG LPTD NGHD SD
Gene ID:	22883
Uniprot:	O94985
Cellular Location:	Cell junction, Cell projection, Endoplasmic reticulum membrane, Golgi apparatus membrane, Nucleus, Single-pass type I membrane protein, postsynaptic cell membrane, synapse
Calculated MW:	108kDa/109kDa
Observed MW:	110kDa

Synonyms:

CLSTN1, ALC-ALPHA, CDHR12, CST-1, CSTN1, PIK3CD, XB31alpha, alcalpha1, alcalpha2

Background:

This gene is a member of the calsyntenin family, a subset of the cadherin superfamily. The encoded transmembrane protein, also known as alcadein-alpha, is thought to bind to kinesin-1 motors to mediate the axonal anterograde transport of certain types of vesicle. Amyloid precursor protein (APP) is trafficked via these vesicles and so this protein is being investigated to see how it might contribute to the mechanisms underlying Alzheimer's disease. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	CLSTN1: Induces KLC1 association with vesicles and functions as a cargo in axonal anterograde transport. Complex formation with APBA2 and APP, stabilizes APP metabolism and enhances APBA2- mediated suppression of beta-APP40 secretion, due to the retardation of intracellular APP maturation. In complex with APBA2 and C99, a C-terminal APP fragment, abolishes C99 interaction with PSEN1 and thus APP C99 cleavage by gamma-secretase, most probably through stabilization of the direct interaction between APBA2 and APP. The intracellular fragment AlcICD suppresses APBB1-dependent transactivation stimulated by APP C-terminal intracellular fragment (AICD), most probably by competing with AICD for APBB1- binding. May modulate calcium-mediated postsynaptic signals. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; Cell surface Chromosomal Location of Human Ortholog: 1p36.22 Cellular Component: Golgi membrane; postsynaptic membrane; endoplasmic reticulum membrane; cell projection; integral to membrane; cell junction; nucleus Molecular Function:protein binding; X11-like protein binding; kinesin binding; beta-amyloid binding; calcium ion binding Biological Process: homophilic cell adhesion; cell adhesion
NCBI Summary:	This gene is a member of the calsyntenin family, a subset of the cadherin superfamily. The encoded transmembrane protein, also known as alcadein-alpha, is thought to bind to kinesin-1 motors to mediate the axonal anterograde transport of certain types of vesicle. Amyloid precursor protein (APP) is trafficked via these vesicles and so this protein is being investigated to see how it might contribute to the mechanisms underlying Alzheimer's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
UniProt Code:	O94985
NCBI GenInfo Identifier:	23396490
NCBI Gene ID:	22883
NCBI Accession:	O94985.1
UniProt Secondary Accession:	O94985,Q5SR52, Q5UE58, Q71MN0, Q8N4K9, A8K183,
UniProt Related Accession:	O94985
Molecular Weight:	108,643 Da
NCBI Full Name:	Calsyntenin-1
NCBI Synonym Full Names:	calsyntenin 1
NCBI Official Symbol:	CLSTN1
NCBI Official Synonym Symbols:	CST-1; CSTN1; CDHR12; PIK3CD; ALC-ALPHA; XB31alpha; alcalpha1; alcalpha2
NCBI Protein Information:	calsyntenin-1; alcadein-alpha; alzheimer-related cadherin-like protein; cadherin-related family member 12; non-classical cadherin XB31alpha
UniProt Protein Name:	Calsyntenin-1
UniProt Synonym Protein Names:	Alcadein-alpha; Alc-alpha; Alzheimer-related cadherin-like protein; Non-classical cadherin XB31alphaCleaved into the following 2 chains:Soluble Alc-alpha; SAlc-alpha; CTF1-alphaAlternative name(s):C-terminal fragment 1-alpha
Protein Family:	Calsyntenin
UniProt Gene Name:	CLSTN1
UniProt Entry Name:	CSTN1_HUMAN

Western blot analysis of extracts of various cell lines, using CLSTN1 antibody (CAB15405) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Antibodies

CLSTN1 Antibody (PACO08471)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)