Cell Biology Antibodies 14
Anti-CLPX Antibody (CAB17088)
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- SKU:
- CAB17088
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-CLPX Antibody (CAB17088) |
| Antibody SKU: | CAB17088 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 500-600 of human CLPX (NP_006651.2). |
| Application: | Western blotting |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse liver, Rat live |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 500-600 of human CLPX (NP_006651.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LPVV VPLH SLDE KTLV QILT EPRN AVIP QYQA LFSM DKCE LNVT EDAL KAIA RLAL ERKT GARG LRSI MEKL LLEP MFEV PNSD IVCV EVDK EVVE GKKE P |
| Cellular Location: | |
| Calculated MW: | |
| Observed MW: | 69kDa |
| Synonyms: | CLPX |
| Background: | The protein encoded by this gene is part of a protease found in mitochondria. This protease is ATP-dependent and targets specific proteins for degradation. The protease consists of two heptameric rings of the CLPP catalytic subunit sandwiched between two hexameric rings of the chaperone subunit encoded by this gene. Targeted proteins are unwound by this protein and then passed on to the CLPP subunit for degradation. Two transcript variants, one protein-coding and the other non-protein coding, have been found for this gene. |
 | Western blot analysis of extracts of various cell lines, using CLPX antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |
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