Cell Biology Antibodies 10
Anti-CLPS Antibody (CAB6364)
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- SKU:
- CAB6364
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-CLPS Antibody |
| Antibody SKU: | CAB6364 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 18-112 of human CLPS (NP_001823.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:10 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Mouse pancreas |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 18-112 of human CLPS (NP_001823.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | APGP RGII INLE NGEL CMNS AQCK SNCC QHSS ALGL ARCT SMAS ENSE CSVK TLYG IYYK CPCE RGLT CEGD KTIV GSIT NTNF GICH DAGR SKQ |
| Gene ID: | 1208 |
| Uniprot: | P04118 |
| Cellular Location: | Secreted |
| Calculated MW: | 11kDa |
| Observed MW: | 12kDa |
| Synonyms: | CLPS, colipase |
| Background: | The protein encoded by this gene is a cofactor needed by pancreatic lipase for efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilizing an active conformation and considerably increasing the overall hydrophobic binding site. The gene product allows lipase to anchor noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. This cofactor is only expressed in pancreatic acinar cells, suggesting regulation of expression by tissue-specific elements. Three transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | CLPS: Colipase is a cofactor of pancreatic lipase. It allows the lipase to anchor itself to the lipid-water interface. Without colipase the enzyme is washed off by bile salts, which have an inhibitory effect on the lipase. Belongs to the colipase family. |
| UniProt Protein Details: | Protein type:Activator; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 6p21.31 Cellular Component: extracellular region Molecular Function:enzyme activator activity Biological Process: positive regulation of catalytic activity; phototransduction, visible light; lipid metabolic process; retinoid metabolic process; lipid catabolic process; lipid digestion |
| NCBI Summary: | The protein encoded by this gene is a cofactor needed by pancreatic lipase for efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilizing an active conformation and considerably increasing the overall hydrophobic binding site. The gene product allows lipase to anchor noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. This cofactor is only expressed in pancreatic acinar cells, suggesting regulation of expression by tissue-specific elements. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] |
| UniProt Code: | P04118 |
| NCBI GenInfo Identifier: | 116900 |
| NCBI Gene ID: | 1208 |
| NCBI Accession: | P04118.2 |
| UniProt Secondary Accession: | P04118,Q5T9G7, Q5U809, |
| UniProt Related Accession: | P04118 |
| Molecular Weight: | 112 |
| NCBI Full Name: | Colipase |
| NCBI Synonym Full Names: | colipase, pancreatic |
| NCBI Official Symbol: | CLPS |
| NCBI Protein Information: | colipase; pancreatic colipase preproprotein |
| UniProt Protein Name: | Colipase |
| Protein Family: | Colipase |
| UniProt Gene Name: | CLPS |
| UniProt Entry Name: | COL_HUMAN |
 | Western blot analysis of extracts of mouse pancreas, using CLPS antibody (CAB6364) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of HeLa cells using CLPS antibody (CAB6364). Blue: DAPI for nuclear staining. |
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