Cell Biology Antibodies 12
Anti-CLPP Antibody (CAB9127)
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- SKU:
- CAB9127
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-CLPP Antibody |
| Antibody SKU: | CAB9127 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-277 of human CLPP (NP_006003.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:1000 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | LO2, A-549, BxPC-3, 22Rv1, Mouse liver, Mouse kidney, Mouse heart, Mouse lung, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-277 of human CLPP (NP_006003.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MWPG ILVG GARV ASCR YPAL GPRL AAHF PAQR PPQR TLQN GLAL QRCL HATA TRAL PLIP IVVE QTGR GERA YDIY SRLL RERI VCVM GPID DSVA SLVI AQLL FLQS ESNK KPIH MYIN SPGG VVTA GLAI YDTM QYIL NPIC TWCV GQAA SMGS LLLA AGTP GMRH SLPN SRIM IHQP SGGA RGQA TDIA IQAE EIMK LKKQ LYNI YAKH TKQS LQVI ESAM ERDR YMSP MEAQ EFGI LDKV LVHP PQDG EDEP TLVQ KEPV EAAP AAEP VPAS T |
| Gene ID: | 8192 |
| Uniprot: | Q16740 |
| Cellular Location: | Mitochondrion matrix |
| Calculated MW: | 30kDa |
| Observed MW: | 30kDa |
| Synonyms: | CLPP, DFNB81, PRLTS3 |
| Background: | The protein encoded by this gene belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane. |
| UniProt Protein Function: | Protease component of the Clp complex that cleaves peptides and various proteins in an ATP-dependent process. Has low peptidase activity in the absence of CLPX. The Clp complex can degrade CSN1S1, CSN2 and CSN3, as well as synthetic peptides (in vitro) and may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. |
| NCBI Summary: | The protein encoded by this gene belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q16740 |
| NCBI GenInfo Identifier: | 5174419 |
| NCBI Gene ID: | 8192 |
| NCBI Accession: | NP_006003 |
| UniProt Secondary Accession: | Q16740,B2R4W5, |
| UniProt Related Accession: | Q16740 |
| Molecular Weight: | 24.2kDa (222aa), confirmed by MALDI-TOF |
| NCBI Full Name: | ATP-dependent Clp protease proteolytic subunit, mitochondrial |
| NCBI Synonym Full Names: | caseinolytic mitochondrial matrix peptidase proteolytic subunit |
| NCBI Official Symbol: | CLPP |
| NCBI Official Synonym Symbols: | DFNB81; PRLTS3 |
| NCBI Protein Information: | ATP-dependent Clp protease proteolytic subunit, mitochondrial |
| UniProt Protein Name: | ATP-dependent Clp protease proteolytic subunit, mitochondrial |
| UniProt Synonym Protein Names: | Endopeptidase Clp |
| Protein Family: | ATP-dependent Clp protease proteolytic |
| UniProt Gene Name: | CLPP |
 | Western blot analysis of extracts of various cell lines, using CLPP antibody (CAB9127) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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