Cell Biology Antibodies 11

Anti-CLK2 Antibody (CAB7885)

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SKU:
CAB7885
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CLK2 Antibody
Antibody SKU:CAB7885
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 239-498 of human CLK2 (NP_003984.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:BT-474, HeLa, Mouse brain, Mouse lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 239-498 of human CLK2 (NP_003984.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:CISF ELLG LSTF DFLK DNNY LPYP IHQV RHMA FQLC QAVK FLHD NKLT HTDL KPEN ILFV NSDY ELTY NLEK KRDE RSVK STAV RVVD FGSA TFDH EHHS TIVS TRHY RAPE VILE LGWS QPCD VWSI GCII FEYY VGFT LFQT HDNR EHLA MMER ILGP IPSR MIRK TRKQ KYFY RGRL DWDE NTSA GRYV RENC KPLR RYLT SEAE EHHQ LFDL IESM LEYE PAKR LTLG EALQ HPFF ARLR AEPP NKLW DSSR DISR
Gene ID:1196
Uniprot:P49760
Cellular Location:Nucleus, Nucleus speckle, Nucleus speckle
Calculated MW:17kDa/59kDa/60kDa
Observed MW:70kDa
Synonyms:CLK2
Background:This gene encodes a dual specificity protein kinase that phosphorylates serine/threonine and tyrosine-containing substrates. Activity of this protein regulates serine- and arginine-rich (SR) proteins of the spliceosomal complex, thereby influencing alternative transcript splicing. Chromosomal translocations have been characterized between this locus and the PAFAH1B3 (platelet-activating factor acetylhydrolase 1b, catalytic subunit 3 (29kDa)) gene on chromosome 19, resulting in the production of a fusion protein. Note that this gene is distinct from the TELO2 gene (GeneID:9894), which shares the CLK2 alias, but encodes a protein that is involved in telomere length regulation. There is a pseudogene for this gene on chromosome 7. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:CLK2: Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Acts as a suppressor of hepatic gluconeogenesis and glucose output by repressing PPARGC1A transcriptional activity on gluconeogenic genes via its phosphorylation. Phosphorylates PPP2R5B thereby stimulating the assembly of PP2A phosphatase with the PPP2R5B-AKT1 complex leading to dephosphorylation of AKT1. Phosphorylates: PTPN1, SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Protein kinase, dual-specificity (non-receptor); Protein kinase, CMGC; EC 2.7.12.1; Kinase, protein; CMGC group; CLK family

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleoplasm; nucleus

Molecular Function:protein binding; protein serine/threonine kinase activity

Biological Process: negative regulation of gluconeogenesis; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of RNA splicing; response to ionizing radiation

NCBI Summary:This gene encodes a dual specificity protein kinase that phosphorylates serine/threonine and tyrosine-containing substrates. Activity of this protein regulates serine- and arginine-rich (SR) proteins of the spliceosomal complex, thereby influencing alternative transcript splicing. Chromosomal translocations have been characterized between this locus and the PAFAH1B3 (platelet-activating factor acetylhydrolase 1b, catalytic subunit 3 (29kDa)) gene on chromosome 19, resulting in the production of a fusion protein. Note that this gene is distinct from the TELO2 gene (GeneID:9894), which shares the CLK2 alias, but encodes a protein that is involved in telomere length regulation. There is a pseudogene for this gene on chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]
UniProt Code:P49760
NCBI GenInfo Identifier:1705919
NCBI Gene ID:1196
NCBI Accession:P49760.1
UniProt Secondary Accession:P49760,Q96CQ0, B1AVS9, B5MBX6,
UniProt Related Accession:P49760
Molecular Weight:59,962 Da
NCBI Full Name:Dual specificity protein kinase CLK2
NCBI Synonym Full Names:CDC like kinase 2
NCBI Official Symbol:CLK2  
NCBI Protein Information:dual specificity protein kinase CLK2
UniProt Protein Name:Dual specificity protein kinase CLK2
UniProt Synonym Protein Names:CDC-like kinase 2
Protein Family:Dual specificity protein kinase
UniProt Gene Name:CLK2  
UniProt Entry Name:CLK2_HUMAN
Anti-CLK2 Antibody (CAB7885)Western blot analysis of extracts of various cell lines, using CLK2 antibody (CAB7885) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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