Name: Anti-CLIP1 Antibody (CAB7722)
Brand: Signal Transduction Antibodies 3
SKU: CAB7722
Availability: OutOfStock

Antibody Name:	Anti-CLIP1 Antibody
Antibody SKU:	CAB7722
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1228-1427 of human CLIP1 (NP_002947.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Mouse lung, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1228-1427 of human CLIP1 (NP_002947.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SALL TEKD AELE KLRN EVTV LRGE NASA KSLH SVVQ TLES DKVK LELK VKNL ELQL KENK RQLS SSSG NTDT QADE DERA QESQ IDFL NSVI VDLQ RKNQ DLKM KVEM MSEA ALNG NGDD LNNY DSDD QEKQ SKKK PRLF CDIC DCFD LHDT EDCP TQAQ MSED PPHS THHG SRGE ERPY CEIC EMFG HWAT NCND DETF
Gene ID:	6249
Uniprot:	P30622
Cellular Location:	Cell projection, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle membrane, Peripheral membrane protein, cytoskeleton, ruffle
Calculated MW:	156kDa/160kDa/162kDa
Observed MW:	162kDa

Synonyms:	CLIP1, CLIP, CLIP-170, CLIP170, CYLN1, RSN
Background:	The protein encoded by this gene links endocytic vesicles to microtubules. This gene is highly expressed in Reed-Sternberg cells of Hodgkin disease. Several transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	Restin: Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth and microtubule bundling. Links cytoplasmic vesicles to microtubules and thereby plays an important role in intracellular vesicle trafficking. Plays a role macropinocytosis and endosome trafficking. Interacts with MTOR; phosphorylates and regulates CLIP1. Interacts (via CAP-Gly domains) with tubulin. Interacts with SLAIN2. Interacts (via zinc finger) with DCTN1. Interacts with MAPRE1 and MAPRE3. Detected in dendritic cells. Highly expressed in the Reed-Sternberg cells of Hodgkin disease. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 12q24.3 Cellular Component: kinetochore; microtubule cytoskeleton; ruffle; microtubule; centrosome; cytoplasmic vesicle membrane; cytoplasmic microtubule; cytoplasm; intermediate filament; cytosol; endosome Molecular Function:tubulin binding; microtubule plus-end binding; protein binding; protein homodimerization activity; nucleic acid binding; zinc ion binding; microtubule binding; metal ion binding Biological Process: mitosis; positive regulation of microtubule polymerization; mitotic cell cycle; microtubule bundle formation
NCBI Summary:	The protein encoded by this gene links endocytic vesicles to microtubules. This gene is highly expressed in Reed-Sternberg cells of Hodgkin disease. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
UniProt Code:	P30622
NCBI GenInfo Identifier:	261260059
NCBI Gene ID:	6249
NCBI Accession:	P30622.2
UniProt Secondary Accession:	P30622,Q17RS4, Q29RG0, A0AVD3,
UniProt Related Accession:	P30622
Molecular Weight:	156,781 Da
NCBI Full Name:	CAP-Gly domain-containing linker protein 1
NCBI Synonym Full Names:	CAP-GLY domain containing linker protein 1
NCBI Official Symbol:	CLIP1
NCBI Official Synonym Symbols:	RSN; CLIP; CYLN1; CLIP170; CLIP-170
NCBI Protein Information:	CAP-Gly domain-containing linker protein 1; cytoplasmic linker protein 1; cytoplasmic linker protein CLIP-170; cytoplasmic linker protein 170 alpha-2; restin (Reed-Steinberg cell-expressed intermediate filament-associated protein)
UniProt Protein Name:	CAP-Gly domain-containing linker protein 1
UniProt Synonym Protein Names:	Cytoplasmic linker protein 1; Cytoplasmic linker protein 170 alpha-2; CLIP-170; Reed-Sternberg intermediate filament-associated protein; Restin
Protein Family:	CAP-Gly domain-containing linker protein
UniProt Gene Name:	CLIP1
UniProt Entry Name:	CLIP1_HUMAN

	Western blot analysis of extracts of various cell lines, using CLIP1 antibody (CAB7722) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse lung using CLIP1 antibody (CAB7722) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat heart using CLIP1 antibody (CAB7722) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using CLIP1 antibody (CAB7722) at dilution of 1:100 (40x lens).