Cell Biology Antibodies 2
Anti-CLDN3 Antibody (CAB11650)
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- SKU:
- CAB11650
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-CLDN3 Antibody |
| Antibody SKU: | CAB11650 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 141-220 of human CLDN3 (NP_001297.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse liver, Mouse lung, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 141-220 of human CLDN3 (NP_001297.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TIIR DFYN PVVP EAQK REMG AGLY VGWA AAAL QLLG GALL CCSC PPRE KKYT ATKV VYSA PRST GPGA SLGT GYDR KDYV |
| Gene ID: | 1365 |
| Uniprot: | O15551 |
| Cellular Location: | Cell junction, Cell membrane, Multi-pass membrane protein, tight junction |
| Calculated MW: | 23kDa |
| Observed MW: | 27kDa |
| Synonyms: | CLDN3, C7orf1, CPE-R2, CPETR2, HRVP1, RVP1, claudin-3 |
| Background: | Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat. |
| UniProt Protein Function: | Claudin-3: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. CLDN3 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Belongs to the claudin family. |
| UniProt Protein Details: | Protein type:Membrane protein, multi-pass; Membrane protein, integral; Cytoskeletal Chromosomal Location of Human Ortholog: 7q11.23 Cellular Component: apicolateral plasma membrane; integral to membrane; integral to plasma membrane; lateral plasma membrane; tight junction Molecular Function:identical protein binding; structural molecule activity; transmembrane receptor activity Biological Process: calcium-independent cell-cell adhesion; protein heterooligomerization; protein homooligomerization; response to hypoxia; signal transduction |
| NCBI Summary: | Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O15551 |
| NCBI GenInfo Identifier: | 6685273 |
| NCBI Gene ID: | 1365 |
| NCBI Accession: | O15551.1 |
| UniProt Secondary Accession: | O15551,Q9Z0G9, Q63400, |
| UniProt Related Accession: | O15551 |
| Molecular Weight: | 23,319 Da |
| NCBI Full Name: | Claudin-3 |
| NCBI Synonym Full Names: | claudin 3 |
| NCBI Official Symbol: | CLDN3 |
| NCBI Official Synonym Symbols: | RVP1; HRVP1; C7orf1; CPE-R2; CPETR2 |
| NCBI Protein Information: | claudin-3 |
| UniProt Protein Name: | Claudin-3 |
| UniProt Synonym Protein Names: | Clostridium perfringens enterotoxin receptor 2; CPE-R 2; CPE-receptor 2; Rat ventral prostate.1 protein homolog; hRVP1 |
| Protein Family: | Claudin |
| UniProt Gene Name: | CLDN3 |
| UniProt Entry Name: | CLD3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CLDN3 antibody (CAB11650) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s. |
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