Name: Anti-CLDN14 Antibody (CAB7787)
Brand: Cell Biology Antibodies 11
SKU: CAB7787
Availability: OutOfStock

Antibody Name:	Anti-CLDN14 Antibody
Antibody SKU:	CAB7787
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-85 of human CLDN14 (NP_652763.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	293T, LO2, A-431, U-87MG

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-85 of human CLDN14 (NP_652763.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TILP HWRR TAHV GTNI LTAV SYLK GLWM ECVW HSTG IYQC QIYR SLLA LPQD LQAA RALM V
Gene ID:	23562
Uniprot:	O95500
Cellular Location:	Cell junction, Cell membrane, Multi-pass membrane protein, tight junction
Calculated MW:	25kDa
Observed MW:	24kDa

Synonyms:

CLDN14, DFNB29

Background:

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. The encoded protein also binds specifically to the WW domain of Yes-associated protein. Defects in this gene are the cause of an autosomal recessive form of nonsyndromic sensorineural deafness. It is also reported that four synonymous variants in this gene are associated with kidney stones and reduced bone mineral density. Several transcript variants encoding the same protein have been found for this gene.

UniProt Protein Function:	Claudin-14: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Defects in CLDN14 are the cause of deafness autosomal recessive type 29 (DFNB29). DFNB29 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Belongs to the claudin family.
UniProt Protein Details:	Protein type:Cell adhesion; Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 21q22.3 Cellular Component: tight junction; endoplasmic reticulum; plasma membrane; integral to membrane Molecular Function:identical protein binding; structural molecule activity Biological Process: intercellular junction assembly and maintenance; protein complex assembly; calcium-independent cell-cell adhesion Disease: Deafness, Autosomal Recessive 29
NCBI Summary:	Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. The encoded protein also binds specifically to the WW domain of Yes-associated protein. Defects in this gene are the cause of an autosomal recessive form of nonsyndromic sensorineural deafness. It is also reported that four synonymous variants in this gene are associated with kidney stones and reduced bone mineral density. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jun 2010]
UniProt Code:	O95500
NCBI GenInfo Identifier:	6685304
NCBI Gene ID:	23562
NCBI Accession:	O95500.1
UniProt Related Accession:	O95500
Molecular Weight:	239
NCBI Full Name:	Claudin-14
NCBI Synonym Full Names:	claudin 14
NCBI Official Symbol:	CLDN14
NCBI Official Synonym Symbols:	DFNB29
NCBI Protein Information:	claudin-14
UniProt Protein Name:	Claudin-14
Protein Family:	Claudin
UniProt Gene Name:	CLDN14
UniProt Entry Name:	CLD14_HUMAN

Western blot analysis of extracts of various cell lines, using CLDN14 antibody (CAB7787) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.

ELISA Kits

Human CLDN14 (Claudin 14) ELISA Kit (HUES01891)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)