Signal Transduction Antibodies 2

Anti-CLCNKA Antibody (CAB3792)

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SKU:
CAB3792
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-CLCNKA Antibody
Antibody SKU:CAB3792
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 470-644 of human CLCNKA (NP_001244068.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:LO2, HT-29, A-549
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 470-644 of human CLCNKA (NP_001244068.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QSCQ PSFY DGTI IVKK LPYL PRIL GRNI GSHH VRVE HFMN HSIT TLAK DTPL EEVV KVVT STDV TEYP LVES TESQ ILVG IVQR AQLV QALQ AEPP SRAP GHQQ CLQD ILAR GCPT EPVT LTLF SETT LHQA QNLF KLLN LQSL FVTS RGRA VGCV SWVE MKKA ISNL TNPP APK
Gene ID:1187
Uniprot:P51800
Cellular Location:Membrane, Multi-pass membrane protein
Calculated MW:70kDa/75kDa
Observed MW:75kDa
Synonyms:CLCNKA, CLCK1, ClC-K1, hClC-Ka
Background:This gene is a member of the CLC family of voltage-gated chloride channels. The encoded protein is predicted to have 12 transmembrane domains, and requires a beta subunit called barttin to form a functional channel. It is thought to function in salt reabsorption in the kidney and potassium recycling in the inner ear. The gene is highly similar to CLCNKB, which is located 10 kb downstream from this gene. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:CLCNKA: Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. May be important in urinary concentrating mechanisms. Defects in CLCNKA are a cause of Bartter syndrome type 4B (BS4B). A digenic, recessive disorder characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. Bartter syndrome type 4B is associated with sensorineural deafness. Belongs to the chloride channel (TC 2.A.49) family. CLCNKA subfamily.
UniProt Protein Details:

Protein type:Transporter, ion channel; Membrane protein, integral; Membrane protein, multi-pass; Transporter

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function:metal ion binding; voltage-gated chloride channel activity

Biological Process: transport; excretion; transmembrane transport

Disease: Bartter Syndrome, Type 4b

NCBI Summary:This gene is a member of the CLC family of voltage-gated chloride channels. The encoded protein is predicted to have 12 transmembrane domains, and requires a beta subunit called barttin to form a functional channel. It is thought to function in salt reabsorption in the kidney and potassium recycling in the inner ear. The gene is highly similar to CLCNKB, which is located 10 kb downstream from this gene. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P51800
NCBI GenInfo Identifier:1705857
NCBI Gene ID:1187
NCBI Accession:P51800.1
UniProt Secondary Accession:P51800,Q5T5P8, Q5T5Q4, Q7Z6D1, Q86VT1, B4DPD3, E7EPH6
UniProt Related Accession:P51800
Molecular Weight:
NCBI Full Name:Chloride channel protein ClC-Ka
NCBI Synonym Full Names:chloride channel, voltage-sensitive Ka
NCBI Official Symbol:CLCNKA  
NCBI Official Synonym Symbols:CLCK1; ClC-K1; hClC-Ka  
NCBI Protein Information:chloride channel protein ClC-Ka; chloride channel Ka; chloride channel, kidney, A
UniProt Protein Name:Chloride channel protein ClC-Ka
UniProt Synonym Protein Names:ClC-K1
Protein Family:Chloride channel protein
UniProt Gene Name:CLCNKA  
UniProt Entry Name:CLCKA_HUMAN
Anti-CLCNKA Antibody (CAB3792)Western blot analysis of extracts of various cell lines, using CLCNKA Antibody (CAB3792) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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