Name: Anti-CLCN4 Antibody (CAB13790)
Brand: Signal Transduction Antibodies 1
SKU: CAB13790
Availability: OutOfStock

Antibody Name:	Anti-CLCN4 Antibody
Antibody SKU:	CAB13790
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 600-700 of human CLCN4 (NP_001821.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, LO2, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 600-700 of human CLCN4 (NP_001821.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MRPR RGEP PLSV LTQD SMTV EDVE TLIK ETDY NGFP VVVS RDSE RLIG FAQR RELI LAIK NARQ RQEG IVSN SIMY FTEE PPEL PANS PHPL KLRR ILNL S
Gene ID:	1183
Uniprot:	P51793
Cellular Location:	Early endosome membrane, Late endosome membrane, Multi-pass membrane protein
Calculated MW:	74kDa/84kDa
Observed MW:	85kDa

Synonyms:

CLCN4, CLC4, ClC-4, ClC-4A, MRX15, MRX49

Background:

The CLCN family of voltage-dependent chloride channel genes comprises nine members (CLCN1-7, Ka and Kb) which demonstrate quite diverse functional characteristics while sharing significant sequence homology. Chloride channel 4 has an evolutionary conserved CpG island and is conserved in both mouse and hamster. This gene is mapped in close proximity to APXL (Apical protein Xenopus laevis-like) and OA1 (Ocular albinism type I), which are both located on the human X chromosome at band p22.3. The physiological role of chloride channel 4 remains unknown but may contribute to the pathogenesis of neuronal disorders. Alternate splicing results in two transcript variants that encode different proteins.

UniProt Protein Function:	CLCN4: Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons. Belongs to the chloride channel (TC 2.A.49) family. ClC-4/CLCN4 subfamily.
UniProt Protein Details:	Protein type:Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ion channel Chromosomal Location of Human Ortholog: Xp22.3 Cellular Component: endosome membrane Molecular Function:antiporter activity; chloride channel activity Biological Process: chloride transport; transport
NCBI Summary:	The CLCN family of voltage-dependent chloride channel genes comprises nine members (CLCN1-7, Ka and Kb) which demonstrate quite diverse functional characteristics while sharing significant sequence homology. Chloride channel 4 has an evolutionary conserved CpG island and is conserved in both mouse and hamster. This gene is mapped in close proximity to APXL (Apical protein Xenopus laevis-like) and OA1 (Ocular albinism type I), which are both located on the human X chromosome at band p22.3. The physiological role of chloride channel 4 remains unknown but may contribute to the pathogenesis of neuronal disorders. Alternate splicing results in two transcript variants that encode different proteins. [provided by RefSeq, Mar 2012]
UniProt Code:	P51793
NCBI GenInfo Identifier:	20141247
NCBI Gene ID:	1183
NCBI Accession:	P51793.2
UniProt Secondary Accession:	P51793,Q9UBU1, A1L3U1, B7Z5Z4,
UniProt Related Accession:	P51793
Molecular Weight:	74,347 Da
NCBI Full Name:	H(+)/Cl(-) exchange transporter 4
NCBI Synonym Full Names:	chloride voltage-gated channel 4
NCBI Official Symbol:	CLCN4
NCBI Official Synonym Symbols:	CLC4; ClC-4; ClC-4A
NCBI Protein Information:	H(+)/Cl(-) exchange transporter 4
UniProt Protein Name:	H(+)/Cl(-) exchange transporter 4
UniProt Synonym Protein Names:	Chloride channel protein 4; ClC-4; Chloride transporter ClC-4
Protein Family:	H(+)/Cl(-) exchange transporter
UniProt Gene Name:	CLCN4
UniProt Entry Name:	CLCN4_HUMAN

Western blot analysis of extracts of various cell lines, using CLCN4 antibody (CAB13790) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Antibodies

CLCN4 Antibody (PACO06354)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)