Cell Biology Antibodies 5

Anti-CLCA1 Antibody (CAB15041)

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SKU:
CAB15041
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CLCA1 Antibody
Antibody SKU:CAB15041
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 22-150 of human CLCA1 (NP_001276.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:mouse uterus, rat small intestine
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 22-150 of human CLCA1 (NP_001276.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:NSLI QLNN NGYE GIVV AIDP NVPE DETL IQQI KDMV TQAS LYLF EATG KRFY FKNV AILI PETW KTKA DYVR PKLE TYKN ADVL VAES TPPG NDEP YTEQ MGNC GEKG ERIH LTPD FIAG KKLA EYGP Q
Gene ID:1179
Uniprot:A8K7I4
Cellular Location:Cell membrane, Extracellular side, Peripheral membrane protein, Secreted, extracellular space
Calculated MW:100kDa
Observed MW:85kDa
Synonyms:CLCA1, CACC, CACC1, CLCRG1, CaCC-1, GOB5, hCLCA1, hCaCC-1
Background:This gene encodes a member of the calcium sensitive chloride conductance protein family. To date, all members of this gene family map to the same region on chromosome 1p31-p22 and share a high degree of homology in size, sequence, and predicted structure, but differ significantly in their tissue distributions. The encoded protein is expressed as a precursor protein that is processed into two cell-surface-associated subunits, although the site at which the precursor is cleaved has not been precisely determined. The encoded protein may be involved in mediating calcium-activated chloride conductance in the intestine.
UniProt Protein Function:CLCA1: May be involved in mediating calcium-activated chloride conductance. May play critical roles in goblet cell metaplasia, mucus hypersecretion, cystic fibrosis and AHR. May be involved in the regulation of mucus production and/or secretion by goblet cells. Involved in the regulation of tissue inflammation in the innate immune response. May play a role as a tumor suppressor. Induces MUC5AC. Belongs to the CLCR family.
UniProt Protein Details:

Protein type:Channel, chloride; Vesicle

Chromosomal Location of Human Ortholog: 1p22.3

Cellular Component: extracellular space; zymogen granule membrane; microvillus; integral to plasma membrane; plasma membrane

Molecular Function:chloride channel activity; metallopeptidase activity; metal ion binding

Biological Process: transport; calcium ion transport; chloride transport; proteolysis; transmembrane transport

NCBI Summary:This gene encodes a member of the calcium sensitive chloride conductance protein family. To date, all members of this gene family map to the same region on chromosome 1p31-p22 and share a high degree of homology in size, sequence, and predicted structure, but differ significantly in their tissue distributions. The encoded protein is expressed as a precursor protein that is processed into two cell-surface-associated subunits, although the site at which the precursor is cleaved has not been precisely determined. The encoded protein may be involved in mediating calcium-activated chloride conductance in the intestine. [provided by RefSeq, Jul 2008]
UniProt Code:A8K7I4
NCBI GenInfo Identifier:110611231
NCBI Gene ID:1179
NCBI Accession:NP_001276.2
UniProt Secondary Accession:A8K7I4,O95151, Q5TDF4, Q9UNF6, Q9UPC6, B2RAV5,
UniProt Related Accession:A8K7I4
Molecular Weight:100,226 Da
NCBI Full Name:calcium-activated chloride channel regulator 1
NCBI Synonym Full Names:chloride channel accessory 1
NCBI Official Symbol:CLCA1  
NCBI Official Synonym Symbols:CACC; GOB5; CACC1; CLCRG1; CaCC-1; hCLCA1; hCaCC-1  
NCBI Protein Information:calcium-activated chloride channel regulator 1; calcium-dependent chloride channel-1; calcium-activated chloride channel protein 1; CLCA family member 1, chloride channel regulator; calcium-activated chloride channel family member 1; chloride channel, calcium activated, family member 1
UniProt Protein Name:Calcium-activated chloride channel regulator 1
UniProt Synonym Protein Names:Calcium-activated chloride channel family member 1; hCLCA1; Calcium-activated chloride channel protein 1; CaCC-1; hCaCC-1
UniProt Gene Name:CLCA1  
UniProt Entry Name:CLCA1_HUMAN
Anti-CLCA1 Antibody (CAB15041)Western blot analysis of extracts of various cell lines, using CLCA1 antibody (CAB15041) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
Anti-CLCA1 Antibody (CAB15041)Immunofluorescence analysis of rat colon using CLCA1 Polyclonal Antibody (CAB15041) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-CLCA1 Antibody (CAB15041)Immunofluorescence analysis of human colon carcinoma using CLCA1 Polyclonal Antibody (CAB15041) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-CLCA1 Antibody (CAB15041)Immunofluorescence analysis of mouse colon using CLCA1 Polyclonal Antibody (CAB15041) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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