Cell Biology Antibodies 12
Anti-Claudin-18 Antibody (CAB8392)
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- SKU:
- CAB8392
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Claudin-18 Antibody |
| Antibody SKU: | CAB8392 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-80 of human CLDN18 (NP_057453.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:1000 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HL-60, 293T, B cells, Mouse liver, Mouse kidney, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-80 of human CLDN18 (NP_057453.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GCIA ATGM DMWS TQDL YDNP VTSV FQYE GLWR SCVR QSSG FTEC RPYF TILG LPAM LQAV R |
| Gene ID: | 51208 |
| Uniprot: | P56856 |
| Cellular Location: | Cell junction, Cell membrane, Multi-pass membrane protein, tight junction |
| Calculated MW: | 27kDa |
| Observed MW: | 28kDa |
| Synonyms: | CLDN18, SFTA5, SFTPJ |
| Background: | This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified. |
| UniProt Protein Function: | Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. |
| NCBI Summary: | This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jun 2010] |
| UniProt Code: | P56856 |
| NCBI GenInfo Identifier: | 7387578 |
| NCBI Gene ID: | 51208 |
| NCBI Accession: | P56856.1 |
| UniProt Secondary Accession: | P56856,Q96PH4, A5PL21, |
| UniProt Related Accession: | P56856 |
| Molecular Weight: | 27,720 Da |
| NCBI Full Name: | Claudin-18 |
| NCBI Synonym Full Names: | claudin 18 |
| NCBI Official Symbol: | CLDN18 |
| NCBI Official Synonym Symbols: | SFTA5; SFTPJ |
| NCBI Protein Information: | claudin-18 |
| UniProt Protein Name: | Claudin-18 |
| Protein Family: | Claudin |
| UniProt Gene Name: | CLDN18 |
| UniProt Entry Name: | CLD18_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CLDN18 antibody (CAB8392) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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