Immunology Antibodies 2

Anti-Claudin 1 Antibody (CAB2196)

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SKU:
CAB2196
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology

Description

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Antibody Name:Anti-Claudin 1 Antibody
Antibody SKU:CAB2196
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human Claudin 1 (NP_066924.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Rat
Positive Samples:SW620
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human Claudin 1 (NP_066924.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MANA GLQL LGFI LAFL GWIG AIVS TALP QWRI YSYA GDNI VTAQ AMYE GLWM SCVS QSTG QIQC KVFD SLLN LSST LQAT RALM VVGI LLGV IAIF VATV GMKC MKCL EDDE VQKM RMAV IGGA IFLL AGLA ILVA TAWY GNRI VQEF YDPM TPVN ARYE FGQA LFTG WAAA SLCL LGGA LLCC SCPR KTTS YPTP RPYP KPAP SSGK DYV
Gene ID:9076
Uniprot:O95832
Cellular Location:Cell junction, Cell membrane, Multi-pass membrane protein, tight junction
Calculated MW:22kDa
Observed MW:20kDa
Synonyms:CLDN1, CLD1, ILVASC, SEMP1, claudin-1, Claudin 1
Background:Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.
UniProt Protein Function:Claudin-1: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Acts as a co- receptor for HCV entry into hepatic cells. Can form homo- and heteropolymers with other CLDN. Homopolymers interact with CLDN3, but not CLDN2, homopolymers. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ and INADL. May interact with HCV E1 and E2 proteins. Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis. Belongs to the claudin family.
UniProt Protein Details:

Protein type:Membrane protein, integral; Cytoskeletal; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 3q28-q29

Cellular Component: integral to membrane; integral to plasma membrane; tight junction

Molecular Function:identical protein binding; protein binding

Biological Process: calcium-independent cell-cell adhesion; intercellular junction assembly and maintenance; protein heterooligomerization; protein homooligomerization

Disease: Ichthyosis, Leukocyte Vacuoles, Alopecia, And Sclerosing Cholangitis

NCBI Summary:Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. [provided by RefSeq, Jul 2008]
UniProt Code:O95832
NCBI GenInfo Identifier:6685283
NCBI Gene ID:9076
NCBI Accession:O95832.1
UniProt Related Accession:O95832
Molecular Weight:22,744 Da
NCBI Full Name:Claudin-1
NCBI Synonym Full Names:claudin 1
NCBI Official Symbol:CLDN1  
NCBI Official Synonym Symbols:CLD1; SEMP1; ILVASC  
NCBI Protein Information:claudin-1
UniProt Protein Name:Claudin-1
UniProt Synonym Protein Names:Senescence-associated epithelial membrane protein
Protein Family:Claudin
UniProt Gene Name:CLDN1  
UniProt Entry Name:CLD1_HUMAN
Anti-Claudin 1 Antibody (CAB2196)Western blot analysis of extracts of SW620 cells, using CLDN1 antibody (CAB2196) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
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