Cell Biology Antibodies 8

Anti-CKMT1B Antibody (CAB3046)

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SKU:
CAB3046
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CKMT1B Antibody
Antibody SKU:CAB3046
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-85 of human CKMT1B (NP_066270.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse brain, Mouse kidney, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-85 of human CKMT1B (NP_066270.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAGP FSRL LSAR PGLR LLAL AGAG SLAA GFLL RPEP VRAA SERR RLYP PSAE YPDL RKHN NCMA SHLT PAVY ARLC DKTT PTGW T
Gene ID:1159
Uniprot:P12532
Cellular Location:Intermembrane side, Mitochondrion inner membrane, Peripheral membrane protein
Calculated MW:47kDa/50kDa
Observed MW:40kDa
Synonyms:CKMT1B, CKMT, CKMT1, UMTCK
Background:Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins.
UniProt Protein Function:Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
NCBI Summary:Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. [provided by RefSeq, Jul 2008]
UniProt Code:P12532
NCBI GenInfo Identifier:125315
NCBI Gene ID:548596
NCBI Accession:P12532.1
UniProt Secondary Accession:P12532,Q0VAM3, Q32NF6, Q53FC4, B4DIT8, B7ZA09,
UniProt Related Accession:P12532
Molecular Weight:50,421 Da
NCBI Full Name:Creatine kinase U-type, mitochondrial
NCBI Synonym Full Names:creatine kinase, mitochondrial 1A
NCBI Official Symbol:CKMT1A  
NCBI Official Synonym Symbols:CKMT1; U-MtCK; mia-CK  
NCBI Protein Information:creatine kinase U-type, mitochondrial
UniProt Protein Name:Creatine kinase U-type, mitochondrial
UniProt Synonym Protein Names:Acidic-type mitochondrial creatine kinase; Mia-CK; Ubiquitous mitochondrial creatine kinase; U-MtCK
UniProt Gene Name:CKMT1A  
UniProt Entry Name:KCRU_HUMAN
Anti-CKMT1B Antibody (CAB3046)Western blot analysis of extracts of various cell lines, using CKMT1B antibody (CAB3046) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Anti-CKMT1B Antibody (CAB3046)Immunohistochemistry of paraffin-embedded human colon using CKMT1B antibody (CAB3046) at dilution of 1:150 (40x lens).
Anti-CKMT1B Antibody (CAB3046)Immunofluorescence analysis of NIH-3T3 cells using CKMT1B Polyclonal Antibody (CAB3046) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-CKMT1B Antibody (CAB3046)Immunofluorescence analysis of U-2 OS cells using CKMT1B Polyclonal Antibody (CAB3046) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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