Name: Anti-CKMT1A Antibody (CAB5233)
Brand: Cell Biology Antibodies 9
SKU: CAB5233
Availability: OutOfStock

Antibody Name:	Anti-CKMT1A Antibody
Antibody SKU:	CAB5233
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 258-417 of human CKMT1A (NP_001015001.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse heart, Rat heart, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 258-417 of human CKMT1A (NP_001015001.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FLIW VNEE DHTR VISM EKGG NMKR VFER FCRG LKEV ERLI QERG WEFM WNER LGYI LTCP SNLG TGLR AGVH IKLP LLSK DSRF PKIL ENLR LQKR GTGG VDTA ATGG VFDI SNLD RLGK SEVE LVQL VIDG VNYL IDCE RRLE RGQD IRIP TPVI HTKH
Gene ID:	548596
Uniprot:	P12532
Cellular Location:	Intermembrane side, Mitochondrion inner membrane, Peripheral membrane protein
Calculated MW:	47kDa/50kDa
Observed MW:	47kDa

Synonyms:

CKMT1A, CKMT1, U-MtCK, mia-CK

Background:

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins.

UniProt Protein Function:	Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
NCBI Summary:	Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. [provided by RefSeq, Jul 2008]
UniProt Code:	P12532
NCBI GenInfo Identifier:	125315
NCBI Gene ID:	548596
NCBI Accession:	P12532.1
UniProt Secondary Accession:	P12532,Q0VAM3, Q32NF6, Q53FC4, B4DIT8, B7ZA09,
UniProt Related Accession:	P12532
Molecular Weight:	50,421 Da
NCBI Full Name:	Creatine kinase U-type, mitochondrial
NCBI Synonym Full Names:	creatine kinase, mitochondrial 1A
NCBI Official Symbol:	CKMT1A
NCBI Official Synonym Symbols:	CKMT1; U-MtCK; mia-CK
NCBI Protein Information:	creatine kinase U-type, mitochondrial
UniProt Protein Name:	Creatine kinase U-type, mitochondrial
UniProt Synonym Protein Names:	Acidic-type mitochondrial creatine kinase; Mia-CK; Ubiquitous mitochondrial creatine kinase; U-MtCK
UniProt Gene Name:	CKMT1A
UniProt Entry Name:	KCRU_HUMAN

Western blot analysis of extracts of various cell lines, using CKMT1A Antibody (CAB5233) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Antibodies

Anti-CKMT1B Antibody (CAB3046)

CKMT1A Antibody (PACO08447)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)