Cell Biology Antibodies 3

Anti-CKB Antibody (CAB12631)

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SKU:
CAB12631
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CKB Antibody
Antibody SKU:CAB12631
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 182-381 of human CKB (NP_001814.2).
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, HT-29, HeLa, Mouse brain, Rat spinal cord
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 182-381 of human CKB (NP_001814.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AEQQ QLID DHFL FDKP VSPL LLAS GMAR DWPD ARGI WHND NKTF LVWV NEED HLRV ISMQ KGGN MKEV FTRF CTGL TQIE TLFK SKDY EFMW NPHL GYIL TCPS NLGT GLRA GVHI KLPN LGKH EKFS EVLK RLRL QKRG TGGV DTAA VGGV FDVS NADR LGFS EVEL VQMV VDGV KLLI EMEQ RLEQ GQAI DDLM PAQK
Gene ID:1152
Uniprot:P12277
Cellular Location:Cytoplasm
Calculated MW:42kDa
Observed MW:43kDa
Synonyms:CKB, B-CK, BCK, CKBB, HEL-211, HEL-S-29
Background:The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.
UniProt Protein Function:Function: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
UniProt Protein Details:

Catalytic activity: ATP + creatine = ADP + phosphocreatine.

Subunit structure: Dimer of identical or non-identical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.

Subcellular location: Cytoplasm.

Sequence similarities: Belongs to the ATP:guanido phosphotransferase family.Contains 1 phosphagen kinase C-terminal domain.Contains 1 phosphagen kinase N-terminal domain.

NCBI Summary:The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq]
UniProt Code:P12277
NCBI GenInfo Identifier:125294
NCBI Gene ID:1152
NCBI Accession:P12277.1
UniProt Secondary Accession:P12277,Q2LE07, Q6FG40, Q9UC66, A8K236, B2R5R4,
UniProt Related Accession:P12277
Molecular Weight:42,644 Da
NCBI Full Name:Creatine kinase B-type
NCBI Synonym Full Names:creatine kinase, brain
NCBI Official Symbol:CKB  
NCBI Official Synonym Symbols:B-CK; CKBB  
NCBI Protein Information:creatine kinase B-type; creatine kinase-B; creatine kinase B chain
UniProt Protein Name:Creatine kinase B-type
UniProt Synonym Protein Names:B-CK; Creatine kinase B chain
Protein Family:Creatine kinase
UniProt Gene Name:CKB  
UniProt Entry Name:KCRB_HUMAN
Anti-CKB Antibody (CAB12631)Western blot analysis of extracts of various cell lines, using CKB antibody (CAB12631) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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