Cell Cycle Antibodies 1

Anti-CIT Antibody (CAB3668)

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SKU:
CAB3668
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-CIT Antibody
Antibody SKU:CAB3668
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1000-1300 of human CIT (NP_001193928.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:U-87MG, HeLa, Jurkat, Mouse testis, Mouse thymus, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1000-1300 of human CIT (NP_001193928.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EDNA ELNN QNFY LSKQ LDEA SGAN DEIV QLRS EVDH LRRE ITER EMQL TSQK QTME ALKT TCTM LEEQ VMDL EALN DELL EKER QWEA WRSV LGDE KSQF ECRV RELQ RMLD TEKQ SRAR ADQR ITES RQVV ELAV KEHK AEIL ALQQ ALKE QKLK AESL SDKL NDLE KKHA MLEM NARS LQQK LETE RELK QRLL EEQA KLQQ QMDL QKNH IFRL TQGL QEAL DRAD LLKT ERSD LEYQ LENI QVLY SHEK VKME GTIS QQTK LIDF LQAK MDQP AKKK KGLF SRRK EDPA LPTQ VPLQ Y
Gene ID:11113
Uniprot:O14578
Cellular Location:Cytoplasm
Calculated MW:54kDa/177kDa/231kDa/236kDa
Observed MW:231kDa
Synonyms:CIT, CITK, CRIK, MCPH17, STK21
Background:This gene encodes a serine/threonine-protein kinase that functions in cell division. Together with the kinesin KIF14, this protein localizes to the central spindle and midbody, and functions to promote efficient cytokinesis. This protein is involved in central nervous system development. Polymorphisms in this gene are associated with bipolar disorder and risk for schizophrenia. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:CRIK: an AGC kinase that is a RHO/RAC effector. Binds to the GTP-bound forms of RHO and RAC1. Required for postnatal hippocampal neurogenesis. Rho/CRIK suppresses differentiation and is associated with induction of KyoT1/2, a LIM domain protein gene implicated in integrin-mediated processes and Notch signaling. Plays an important role in the regulation of cytokinesis and the development of the central nervous system. Overexpressed in mantle-cell lymphoma (MCL), an aggressive type of B-cell lymphoma. Three alternatively spliced isoforms have been described.
UniProt Protein Details:

Protein type:Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; DMPK family

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: ruffle; Golgi cisterna; membrane; cell soma; plasma membrane; vacuole; actin cytoskeleton

Molecular Function:protein serine/threonine kinase activity; protein binding; Rho GTPase binding; metal ion binding; SH3 domain binding; ATP binding; PDZ domain binding

Biological Process: positive regulation of cytokinesis; mitosis; mitotic sister chromatid segregation; regulation of actin polymerization and/or depolymerization; cytokinesis; negative regulation of dendrite morphogenesis; protein amino acid phosphorylation; generation of neurons; regulation of catalytic activity; dendrite development; spermatogenesis; G2/M transition of mitotic cell cycle; Golgi organization and biogenesis; mitotic metaphase/anaphase transition

NCBI Summary:This gene encodes a serine/threonine-protein kinase that functions in cell division. Together with the kinesin KIF14, this protein localizes to the central spindle and midbody, and functions to promote efficient cytokinesis. This protein is involved in central nervous system development. Polymorphisms in this gene are associated with bipolar disorder and risk for schizophrenia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]
UniProt Code:O14578
NCBI GenInfo Identifier:57015279
NCBI Gene ID:11113
NCBI Accession:O14578.2
UniProt Secondary Accession:O14578,Q2M5E1, Q6XUH8, Q86UQ9, Q9UPZ7,
UniProt Related Accession:O14578
Molecular Weight:2027
NCBI Full Name:Citron Rho-interacting kinase
NCBI Synonym Full Names:citron rho-interacting serine/threonine kinase
NCBI Official Symbol:CIT  
NCBI Official Synonym Symbols:CRIK; STK21  
NCBI Protein Information:citron Rho-interacting kinase; serine/threonine kinase 21; serine/threonine-protein kinase 21; citron (rho-interacting, serine/threonine kinase 21)
UniProt Protein Name:Citron Rho-interacting kinase
UniProt Synonym Protein Names:Serine/threonine-protein kinase 21
Protein Family:Citrate
UniProt Gene Name:CIT  
UniProt Entry Name:CTRO_HUMAN
Anti-CIT Antibody (CAB3668)Western blot analysis of extracts of various cell lines, using CIT antibody (CAB3668) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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