Autophagy Antibodies

Anti-CISD2 Antibody (CAB14168)

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SKU:
CAB14168
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Autophagy

Description

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Antibody Name:Anti-CISD2 Antibody
Antibody SKU:CAB14168
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 61-135 of human CISD2 (NP_001008389.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, A-549, K-562, Mouse pancreas
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 61-135 of human CISD2 (NP_001008389.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PKKK QQKD SLIN LKIQ KENP KVVN EINI EDLC LTKA AYCR CWRS KTFP ACDG SHNK HNEL TGDN VGPL ILKK KEV
Gene ID:493856
Uniprot:Q8N5K1
Cellular Location:Endoplasmic reticulum membrane, Mitochondrion outer membrane, Single-pass membrane protein
Calculated MW:15kDa
Observed MW:15kDa
Synonyms:CISD2, ERIS, Miner1, NAF-1, WFS2, ZCD2
Background:The protein encoded by this gene is a zinc finger protein that localizes to the endoplasmic reticulum. The encoded protein binds an iron/sulfur cluster and may be involved in calcium homeostasis. Defects in this gene are a cause of Wolfram syndrome 2.
UniProt Protein Function:CISD2: a single-pass membrane protein of the endoplasmic reticulum that regulates autophagy. Has also been reported to localize to the mitochondrion outer membrane. Antagonizes ATG6-mediated cellular autophagy. Participates in the interaction of BCL2 with BECN1 and is required for BCL2-mediated depression of endoplasmic reticulum Ca2+ stores during autophagy. Contributes to BIK-initiated autophagy, while it is not involved in BIK-dependent activation of caspases. Involved in life span control, probably via its function as regulator of autophagy. Directly interacts with BCL2; the interaction is disrupted by BIK interaction with BCL2. It has been reported to mainly localizes to the mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome 2 (WFS2), is a rare autosomal recessive disorder characterized by optic atrophy and diabetes mellitus. Other symptoms include the presence of profound upper gastrointestinal ulceration, significant bleeding tendency, defective platelet aggregation with collagen and various neurological symptoms. Initially thought to be a zinc-finger protein, it was later shown to bind 1 2Fe-2S cluster instead.
UniProt Protein Details:

Protein type:Membrane protein, integral; Endoplasmic reticulum; Autophagy

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: mitochondrial outer membrane; endoplasmic reticulum membrane; protein complex; membrane; endoplasmic reticulum; integral to membrane

Molecular Function:2 iron, 2 sulfur cluster binding; protein binding; protein homodimerization activity; metal ion binding

Biological Process: mitochondrion degradation; multicellular organismal aging; regulation of autophagy

Disease: Wolfram Syndrome 2

NCBI Summary:The protein encoded by this gene is a zinc finger protein that localizes to the endoplasmic reticulum. The encoded protein binds an iron/sulfur cluster and may be involved in calcium homeostasis. Defects in this gene are a cause of Wolfram syndrome 2. [provided by RefSeq, Mar 2011]
UniProt Code:Q8N5K1
NCBI GenInfo Identifier:74729013
NCBI Gene ID:493856
NCBI Accession:Q8N5K1.1
UniProt Secondary Accession:Q8N5K1,Q7Z3D5,
UniProt Related Accession:Q8N5K1
Molecular Weight:135
NCBI Full Name:CDGSH iron-sulfur domain-containing protein 2
NCBI Synonym Full Names:CDGSH iron sulfur domain 2
NCBI Official Symbol:CISD2  
NCBI Official Synonym Symbols:ERIS; WFS2; ZCD2; NAF-1; Miner1  
NCBI Protein Information:CDGSH iron-sulfur domain-containing protein 2; mitoNEET-related 1 protein; zinc finger, CDGSH-type domain 2; nutrient-deprivation autophagy factor-1; endoplasmic reticulum intermembrane small protein
UniProt Protein Name:CDGSH iron-sulfur domain-containing protein 2
UniProt Synonym Protein Names:Endoplasmic reticulum intermembrane small protein; MitoNEET-related 1 protein; Miner1; Nutrient-deprivation autophagy factor-1; NAF-1
Protein Family:CDGSH iron-sulfur domain-containing protein
UniProt Gene Name:CISD2  
UniProt Entry Name:CISD2_HUMAN
Anti-CISD2 Antibody (CAB14168)Western blot analysis of extracts of various cell lines, using CISD2 antibody (CAB14168) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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