KO Validated Antibodies 1

Anti-CIRBP Antibody (CAB6559)[KO Validated]

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SKU:
CAB6559
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-CIRBP Antibody [KO Validated]
Antibody SKU:CAB6559
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human CIRBP (NP_001271.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:10 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:293T, Jurkat, HepG2, U-251MG, Mouse brain, Mouse testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human CIRBP (NP_001271.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MASD EGKL FVGG LSFD TNEQ SLEQ VFSK YGQI SEVV VVKD RETQ RSRG FGFV TFEN IDDA KDAM MAMN GKSV DGRQ IRVD QAGK SSDN RSRG YRGG SAGG RGFF RGGR GRGR GFSR GGGD RGYG GNRF ESRS GGYG GSRD YYSS RSQS GGYS DRSS GGSY RDSY DSYA THNE
Gene ID:1153
Uniprot:Q14011
Cellular Location:Cytoplasm, Nucleus, nucleoplasm
Calculated MW:18kDa/26kDa/28kDa
Observed MW:18kDa
Synonyms:CIRBP, CIRP
Background:
UniProt Protein Function:Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor. Promotes assembly of stress granules (SGs), when overexpressed.
UniProt Code:Q14011
NCBI GenInfo Identifier:5921786
NCBI Gene ID:1153
NCBI Accession:Q14011.1
UniProt Secondary Accession:Q14011,B3KT17, B4E2X2,
UniProt Related Accession:Q14011
Molecular Weight:19kDa
NCBI Full Name:Cold-inducible RNA-binding protein
NCBI Synonym Full Names:cold inducible RNA binding protein
NCBI Official Symbol:CIRBP  
NCBI Official Synonym Symbols:CIRP  
NCBI Protein Information:cold-inducible RNA-binding protein
UniProt Protein Name:Cold-inducible RNA-binding protein
UniProt Synonym Protein Names:A18 hnRNP; Glycine-rich RNA-binding protein CIRP
Protein Family:Cold-inducible RNA-binding protein
UniProt Gene Name:CIRBP  
Anti-CIRBP Antibody [KO Validated] (CAB6559)Western blot analysis of extracts of various cell lines, using CIRBP antibody (CAB6559) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-CIRBP Antibody [KO Validated] (CAB6559)Western blot analysis of extracts from normal (control) and CIRBP knockout (KO) 293T cells, using CIRBP antibody (CAB6559) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
Anti-CIRBP Antibody [KO Validated] (CAB6559)Immunohistochemistry of paraffin-embedded rat testis using CIRBP antibody (CAB6559) at dilution of 1:100 (40x lens).
Anti-CIRBP Antibody [KO Validated] (CAB6559)Immunohistochemistry of paraffin-embedded human breast cancer using CIRBP antibody (CAB6559) at dilution of 1:100 (40x lens).
Anti-CIRBP Antibody [KO Validated] (CAB6559)Immunohistochemistry of paraffin-embedded mouse testis using CIRBP antibody (CAB6559) at dilution of 1:100 (20x lens).
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