Cardiovascular Antibodies

Anti-CIB1 Antibody (CAB4430)

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SKU:
CAB4430
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cardiovascular

Description

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Antibody Name:Anti-CIB1 Antibody
Antibody SKU:CAB4430
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human CIB1 (NP_006375.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, HeLa, DU145, A-549, HT-29, Mouse kidney, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human CIB1 (NP_006375.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGGS GSRL SKEL LAEY QDLT FLTK QEIL LAHR RFCE LLPQ EQRS VESS LRAQ VPFE QILS LPEL KANP FKER ICRV FSTS PAKD SLSF EDFL DLLS VFSD TATP DIKS HYAF RIFD FDDD GTLN REDL SRLV NCLT GEGE DTRL SASE MKQL IDNI LEES DIDR DGTI NLSE FQHV ISRS PDFA SSFK IVL
Gene ID:10519
Uniprot:Q99828
Cellular Location:Apical cell membrane, Cell membrane, Cell projection, Cytoplasm, Golgi apparatus, Lipid-anchor, Membrane, Nucleus, centrosome, cytoskeleton, filopodium tip, growth cone, lamellipodium, microtubule organizing center, perinuclear region, ruffle membrane, sarcolemma, trans-Golgi network
Calculated MW:21kDa/26kDa
Observed MW:22kDa
Synonyms:CIB1, CIB, CIBP, KIP1, PRKDCIP, SIP2-28
Background:This gene encodes a member of the EF-hand domain-containing calcium-binding superfamily. The encoded protein interacts with many other proteins, including the platelet integrin alpha-IIb-beta-3, DNA-dependent protein kinase, presenilin-2, focal adhesion kinase, p21 activated kinase, and protein kinase D. The encoded protein may be involved in cell survival and proliferation, and is associated with several disease states including cancer and Alzheimer's disease. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:CIB1: May convert the inactive conformation of integrin alpha- IIb/beta3 to an active form through binding to the integrin cytoplasmic domain. Induces cell migration and spreading mediated through integrin (possibly via focal adhesion complexes). Functions as a negative regulator of stress activated MAP kinase (MAPK) signaling pathways. May play a role in regulation of apoptosis. Interacts with and up-regulates PTK2/FAK1 activity. Down regulates inositol 1,4,5-trisphosphate receptor-dependent calcium signaling. Participates in endomitotic cell cycle, a form of mitosis in which both karyokinesis and cytokinesis are interrupted and is a hallmark of megakaryocyte differentiation.
UniProt Protein Details:

Protein type:Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q25.3-q26

Cellular Component: Golgi apparatus; filopodium tip; centrosome; neuron projection; endoplasmic reticulum; nucleoplasm; growth cone; membrane; cell soma; perinuclear region of cytoplasm; lamellipodium; apical plasma membrane; cytoplasm; plasma membrane; nucleus; vesicle; sarcolemma

Molecular Function:protein binding; Ras GTPase binding; protein anchor; calcium ion binding

Biological Process: apoptosis; endomitotic cell cycle; negative regulation of megakaryocyte differentiation; cytoplasmic microtubule organization and biogenesis; activation of NF-kappaB transcription factor; negative regulation of cell proliferation; negative regulation of protein amino acid phosphorylation; double-strand break repair; positive regulation of cell proliferation; angiogenesis; positive regulation of cell-matrix adhesion; cell adhesion; negative regulation of protein kinase B signaling cascade; negative regulation of microtubule depolymerization; positive regulation of cell adhesion mediated by integrin; positive regulation of metalloenzyme activity; positive regulation of cell growth; regulation of cell proliferation; cell division; positive regulation of protein amino acid phosphorylation; platelet formation; response to DNA damage stimulus; regulation of cell division; spermatid development; negative regulation of apoptosis; positive regulation of cell migration

NCBI Summary:This gene encodes a member of the EF-hand domain-containing calcium-binding superfamily. The encoded protein interacts with many other proteins, including the platelet integrin alpha-IIb-beta-3, DNA-dependent protein kinase, presenilin-2, focal adhesion kinase, p21 activated kinase, and protein kinase D. The encoded protein may be involved in cell survival and proliferation, and is associated with several disease states including cancer and Alzheimer's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2013]
UniProt Code:Q99828
NCBI GenInfo Identifier:134047806
NCBI Gene ID:10519
NCBI Accession:Q99828.4
UniProt Secondary Accession:Q99828,O00693, O00735, Q6IB49, Q96J54, Q99971, B5BU40 H6WJF3,
UniProt Related Accession:Q99828
Molecular Weight:26,032 Da
NCBI Full Name:Calcium and integrin-binding protein 1
NCBI Synonym Full Names:calcium and integrin binding 1 (calmyrin)
NCBI Official Symbol:CIB1  
NCBI Official Synonym Symbols:CIB; CIBP; KIP1; PRKDCIP; SIP2-28  
NCBI Protein Information:calcium and integrin-binding protein 1; DNA-PK interaction protein; DNA-PKcs-interacting protein; SNK-interacting protein 2-28; DNA-dependent protein kinase interacting protein
UniProt Protein Name:Calcium and integrin-binding protein 1
UniProt Synonym Protein Names:Calcium- and integrin-binding protein; CIBP; Calmyrin; DNA-PKcs-interacting protein; Kinase-interacting protein; KIP; SNK-interacting protein 2-28; SIP2-28
Protein Family:Calcium and integrin-binding protein
UniProt Gene Name:CIB1  
UniProt Entry Name:CIB1_HUMAN
Anti-CIB1 Antibody (CAB4430)Western blot analysis of extracts of various cell lines, using CIB1 antibody (CAB4430) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-CIB1 Antibody (CAB4430)Immunohistochemistry of paraffin-embedded human prostate using CIB1 antibody (CAB4430) at dilution of 1:100 (40x lens).
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