Name: Anti-CHST6 Antibody (CAB10229)
Brand: Metabolism Antibodies 1
SKU: CAB10229
Availability: OutOfStock

Antibody Name:	Anti-CHST6 Antibody
Antibody SKU:	CAB10229
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 196-395 of human CHST6 (NP_067628.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, MCF7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 196-395 of human CHST6 (NP_067628.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RIVH LVRD PRAV LRSR EQTA KALA RDNG IVLG TNGT WVEA DPGL RVVR EVCR SHVR IAEA ATLK PPPF LRGR YRLV RFED LARE PLAE IRAL YAFT GLSL TPQL EAWI HNIT HGSG PGAR REAF KTSS RNAL NVSQ AWRH ALPF AKIR RVQE LCAG ALQL LGYR PVYS EDEQ RNLA LDLV LPRG LNGF TWAS STAS HPRN
Gene ID:	4166
Uniprot:	Q9GZX3
Cellular Location:	Golgi apparatus membrane, Single-pass type II membrane protein
Calculated MW:	44kDa
Observed MW:	44kDa

Synonyms:	CHST6, MCDC1
Background:	The protein encoded by this gene is an enzyme that catalyzes the transfer of a sulfate group to the GlcNAc residues of keratan. Keratan sulfate helps maintain corneal transparency. Defects in this gene are a cause of macular corneal dystrophy (MCD).

UniProt Protein Function:	CHST6: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues of keratan. Mediates sulfation of keratan in cornea. Keratan sulfate plays a central role in maintaining corneal transparency. Acts on the non-reducing terminal GlcNAc of short and long carbohydrate substrates that have poly-N- acetyllactosamine structures. Defects in CHST6 are the cause of macular corneal dystrophy (MCD). MCD is an autosomal recessive disease characterized by corneal opacities. Onset occurs in the first decade, usually between ages 5 and 9. The disorder is progressive. Minute, gray, punctate opacities develop. Corneal sensitivity is usually reduced. Painful attacks with photophobia, foreign body sensations, and recurrent erosions occur in most patients. There are different types of MCD: MCD type I, in which there is a virtual absence of sulfated keratan sulfate (KS) in the serum and cornea, as determined by KS-specific antibodies; and MCD type II, in which the normal sulfated KS-antibody response is present in cornea and serum. MCD type I patients usually have a homozygous missense mutation, while MCD type II patients show a large deletion and replacement in the upstream region of CHST6. The only missense mutation for type II is Cys-50, which is heterozygous with a replacement in the upstream region on the other allele of CHST6. Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.
UniProt Protein Details:	Protein type:Glycan Metabolism - keratan sulfate biosynthesis; Transferase; Membrane protein, integral; EC 2.8.2.- Chromosomal Location of Human Ortholog: 16q22 Cellular Component: Golgi apparatus; Golgi membrane; integral to membrane Molecular Function:N-acetylglucosamine 6-O-sulfotransferase activity Biological Process: keratan sulfate biosynthetic process; N-acetylglucosamine metabolic process; sulfur metabolic process Disease: Macular Dystrophy, Corneal, 1
NCBI Summary:	The protein encoded by this gene is an enzyme that catalyzes the transfer of a sulfate group to the GlcNAc residues of keratan. Keratan sulfate helps maintain corneal transparency. Defects in this gene are a cause of macular corneal dystrophy (MCD). [provided by RefSeq, Jan 2010]
UniProt Code:	Q9GZX3
NCBI GenInfo Identifier:	61212105
NCBI Gene ID:	4166
NCBI Accession:	Q9GZX3.1
UniProt Secondary Accession:	Q9GZX3,D3DUK3,
UniProt Related Accession:	Q9GZX3
Molecular Weight:	44,099 Da
NCBI Full Name:	Carbohydrate sulfotransferase 6
NCBI Synonym Full Names:	carbohydrate sulfotransferase 6
NCBI Official Symbol:	CHST6
NCBI Official Synonym Symbols:	MCDC1
NCBI Protein Information:	carbohydrate sulfotransferase 6
UniProt Protein Name:	Carbohydrate sulfotransferase 6
UniProt Synonym Protein Names:	Corneal N-acetylglucosamine-6-O-sulfotransferase; C-GlcNAc6ST; hCGn6ST; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4-beta; GST4-beta; N-acetylglucosamine 6-O-sulfotransferase 5; GlcNAc6ST-5; Gn6st-5
Protein Family:	Carbohydrate sulfotransferase
UniProt Gene Name:	CHST6
UniProt Entry Name:	CHST6_HUMAN

Western blot analysis of extracts of various cell lines, using CHST6 antibody (CAB10229) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

CHST6 Antibody (PACO04304)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)