Name: Anti-CHST2 Antibody (CAB15755)
Brand: Metabolism Antibodies 1
SKU: CAB15755
Availability: OutOfStock

Antibody Name:	Anti-CHST2 Antibody
Antibody SKU:	CAB15755
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 231-530 of human CHST2 (NP_004258.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	U-251MG

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 231-530 of human CHST2 (NP_004258.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FQLY SPAG SGGR NLTT LGIF GAAT NKVV CSSP LCPA YRKE VVGL VDDR VCKK CPPQ RLAR FEEE CRKY RTLV IKGV RVFD VAVL APLL RDPA LDLK VIHL VRDP RAVA SSRI RSRH GLIR ESLQ VVRS RDPR AHRM PFLE AAGH KLGA KKEG VGGP ADYH ALGA MEVI CNSM AKTL QTAL QPPD WLQG HYLV VRYE DLVG DPVK TLRR VYDF VGLL VSPE MEQF ALNM TSGS GSSS KPFV VSAR NATQ AANA WRTA LTFQ QIKQ VEEF CYQP MAVL GYER VNSP EEVK DLSK TLLR KPRL
Gene ID:	9435
Uniprot:	Q9Y4C5
Cellular Location:	Golgi apparatus, Single-pass type II membrane protein, trans-Golgi network membrane
Calculated MW:	52kDa/57kDa
Observed MW:	60kDa

Synonyms:	CHST2, C6ST, GST-2, GST2, Gn6ST-1, HEL-S-75, glcNAc6ST-1
Background:	This locus encodes a sulfotransferase protein. The encoded enzyme catalyzes the sulfation of a nonreducing N-acetylglucosamine residue, and may play a role in biosynthesis of 6-sulfosialyl Lewis X antigen.

UniProt Protein Function:	CHST2: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches. Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains. Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily. 2 isoforms of the human protein are produced by alternative initiation.
UniProt Protein Details:	Protein type:EC 2.8.2.-; Membrane protein, integral; Transferase; Glycan Metabolism - keratan sulfate biosynthesis Chromosomal Location of Human Ortholog: 3q24 Cellular Component: Golgi membrane; integral to membrane; trans-Golgi network Molecular Function:N-acetylglucosamine 6-O-sulfotransferase activity; sulfotransferase activity Biological Process: keratan sulfate biosynthetic process; multicellular organismal development; N-acetylglucosamine metabolic process; sulfur metabolic process
NCBI Summary:	This locus encodes a sulfotransferase protein. The encoded enzyme catalyzes the sulfation of a nonreducing N-acetylglucosamine residue, and may play a role in biosynthesis of 6-sulfosialyl Lewis X antigen. [provided by RefSeq, Aug 2011]
UniProt Code:	Q9Y4C5
NCBI GenInfo Identifier:	61212252
NCBI Gene ID:	9435
NCBI Accession:	Q9Y4C5.2
UniProt Secondary Accession:	Q9Y4C5,Q2M370, Q9GZN5, Q9UED5, Q9Y6F2, D3DNG5,
UniProt Related Accession:	Q9Y4C5
Molecular Weight:	52,787 Da
NCBI Full Name:	Carbohydrate sulfotransferase 2
NCBI Synonym Full Names:	carbohydrate sulfotransferase 2
NCBI Official Symbol:	CHST2
NCBI Official Synonym Symbols:	C6ST; GST2; GST-2; Gn6ST-1; HEL-S-75; glcNAc6ST-1
NCBI Protein Information:	carbohydrate sulfotransferase 2
UniProt Protein Name:	Carbohydrate sulfotransferase 2
UniProt Synonym Protein Names:	Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2; GST-2; N-acetylglucosamine 6-O-sulfotransferase 1; GlcNAc6ST-1; Gn6ST-1
Protein Family:	Carbohydrate sulfotransferase
UniProt Gene Name:	CHST2
UniProt Entry Name:	CHST2_HUMAN

Western blot analysis of extracts of U-251MG cells, using CHST2 antibody (CAB15755) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Antibodies

CHST2 Antibody (PACO00601)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)