Metabolism Antibodies 1
Anti-CHST1 Antibody (CAB13057)
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- SKU:
- CAB13057
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-CHST1 Antibody |
| Antibody SKU: | CAB13057 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 62-411 of human CHST1 (NP_003645.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:1000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse liver, Mouse kidney, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 62-411 of human CHST1 (NP_003645.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | HILI LATT RSGS SFVG QLFN QHLD VFYL FEPL YHVQ NTLI PRFT QGKS PADR RVML GASR DLLR SLYD CDLY FLEN YIKP PPVN HTTD RIFR RGAS RVLC SRPV CDPP GPAD LVLE EGDC VRKC GLLN LTVA AEAC RERS HVAI KTVR VPEV NDLR ALVE DPRL NLKV IQLV RDPR GILA SRSE TFRD TYRL WRLW YGTG RKPY NLDV TQLT TVCE DFSN SVST GLMR PPWL KGKY MLVR YEDL ARNP MKKT EEIY GFLG IPLD SHVA RWIQ NNTR GDPT LGKH KYGT VRNS AATA EKWR FRLS YDIV AFAQ NACQ QVLA QLGY KIAA SEEE LKNP SVSL VEER DFRP FS |
| Gene ID: | 8534 |
| Uniprot: | O43916 |
| Cellular Location: | Golgi apparatus membrane, Single-pass type II membrane protein |
| Calculated MW: | 46kDa |
| Observed MW: | 47kDa |
| Synonyms: | CHST1, C6ST, GST-1, KS6ST, KSGal6ST, KSST |
| Background: | This locus encodes a member of the keratin sulfotransferase family of proteins. The encoded enzyme catalyzes the sulfation of the proteoglycan keratin. |
| UniProt Protein Function: | CHST1: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. May function in the sulfation of sialyl N- acetyllactosamine oligosaccharide chains attached to glycoproteins. Participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily. |
| UniProt Protein Details: | Protein type:EC 2.8.2.21; Membrane protein, integral; Glycan Metabolism - keratan sulfate biosynthesis; Transferase Chromosomal Location of Human Ortholog: 11p11.2 Cellular Component: Golgi membrane Molecular Function:keratan sulfotransferase activity; N-acetylglucosamine 6-O-sulfotransferase activity; sulfotransferase activity Biological Process: galactose metabolic process; keratan sulfate biosynthetic process; keratan sulfate metabolic process; polysaccharide metabolic process; sulfur metabolic process |
| NCBI Summary: | This locus encodes a member of the keratin sulfotransferase family of proteins. The encoded enzyme catalyzes the sulfation of the proteoglycan keratin. [provided by RefSeq, Aug 2011] |
| UniProt Code: | O43916 |
| NCBI GenInfo Identifier: | 61211700 |
| NCBI Gene ID: | 8534 |
| NCBI Accession: | O43916.1 |
| UniProt Secondary Accession: | O43916,D3DQP2, |
| UniProt Related Accession: | O43916 |
| Molecular Weight: | 46,715 Da |
| NCBI Full Name: | Carbohydrate sulfotransferase 1 |
| NCBI Synonym Full Names: | carbohydrate sulfotransferase 1 |
| NCBI Official Symbol: | CHST1 |
| NCBI Official Synonym Symbols: | C6ST; KSST; GST-1; KS6ST; KSGal6ST |
| NCBI Protein Information: | carbohydrate sulfotransferase 1 |
| UniProt Protein Name: | Carbohydrate sulfotransferase 1 |
| UniProt Synonym Protein Names: | Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1; GST-1; Keratan sulfate Gal-6 sulfotransferase; KS6ST; KSGal6ST; KSST |
| Protein Family: | Carbohydrate sulfotransferase |
| UniProt Gene Name: | CHST1 |
| UniProt Entry Name: | CHST1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CHST1 antibody (CAB13057) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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