Signal Transduction Antibodies 1

Anti-CHRNB3 Antibody (CAB10108)

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SKU:
CAB10108
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-CHRNB3 Antibody
Antibody SKU:CAB10108
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 25-232 of human CHRNB3 (NP_000740.1).
Application:WB
Recommended Dilution:WB 1:1000 - 1:4000
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse brain, Mouse eye, Mouse liver, Mouse skeletal muscle, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 25-232 of human CHRNB3 (NP_000740.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:IAEN EDAL LRHL FQGY QKWV RPVL HSND TIKV YFGL KISQ LVDV DEKN QLMT TNVW LKQE WTDH KLRW NPDD YGGI HSIK VPSE SLWL PDIV LFEN ADGR FEGS LMTK VIVK SNGT VVWT PPAS YKSS CTMD VTFF PFDR QNCS MKFG SWTY DGTM VDLI LINE NVDR KDFF DNGE WEIL NAKG MKGN RRDG VYSY PFIT YSFV LRRL
Gene ID:1142
Uniprot:Q05901
Cellular Location:Cell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse
Calculated MW:52kDa
Observed MW:55kDa
Synonyms:CHRNB3
Background:
UniProt Protein Function:nAChRB3: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta- 3/CHRNB3 sub-subfamily.
UniProt Protein Details:

Protein type:Channel, cation; Membrane protein, integral; Membrane protein, multi-pass; Channel, ligand-gated

Chromosomal Location of Human Ortholog: 8p11.2

Cellular Component: plasma membrane

Molecular Function:acetylcholine binding; acetylcholine receptor activity; channel activity; ligand-gated ion channel activity

Biological Process: neuromuscular synaptic transmission; response to nicotine; signal transduction; synaptic transmission, cholinergic; transport

NCBI Summary:The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are (hetero)pentamers composed of homologous subunits. The subunits that make up the muscle and neuronal forms of nAChRs are encoded by separate genes and have different primary structure. There are several subtypes of neuronal nAChRs that vary based on which homologous subunits are arranged around the central channel. They are classified as alpha-subunits if, like muscle alpha-1 (MIM 100690), they have a pair of adjacent cysteines as part of the presumed acetylcholine binding site. Subunits lacking these cysteine residues are classified as beta-subunits (Groot Kormelink and Luyten, 1997 [PubMed 9009220]). Elliott et al. (1996) [PubMed 8906617] stated that the proposed structure for each subunit is a conserved N-terminal extracellular domain followed by 3 conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region.[supplied by OMIM, Apr 2010]
UniProt Code:Q05901
NCBI GenInfo Identifier:2506128
NCBI Gene ID:1142
NCBI Accession:Q05901.2
UniProt Secondary Accession:Q05901,Q15827,
UniProt Related Accession:Q05901
Molecular Weight:52,729 Da
NCBI Full Name:Neuronal acetylcholine receptor subunit beta-3
NCBI Synonym Full Names:cholinergic receptor nicotinic beta 3 subunit
NCBI Official Symbol:CHRNB3  
NCBI Protein Information:neuronal acetylcholine receptor subunit beta-3
UniProt Protein Name:Neuronal acetylcholine receptor subunit beta-3
Protein Family:Neuronal acetylcholine receptor
UniProt Gene Name:CHRNB3  
UniProt Entry Name:ACHB3_HUMAN
Anti-CHRNB3 Antibody (CAB10108)Western blot analysis of extracts of various cell lines, using CHRNB3 antibody (CAB10108) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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system_update_altDatasheetHuman CHRNB3 Recombinant Protein Neuronal acetylcholine receptor subunit beta-3(CHRNB3) is a cell membrane protein and belongs to the ligand-gated ion channel (TC 1.A.9)