Signal Transduction Antibodies 2
Anti-CHRNA4 Antibody (CAB1648)
- SKU:
- CAB1648
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-CHRNA4 Antibody |
| Antibody SKU: | CAB1648 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of human CHRNA4 |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse |
| Positive Samples: | Mouse kidney |
| Immunogen: | Recombinant protein of human CHRNA4 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 1137 |
| Uniprot: | P43681 |
| Cellular Location: | Cell junction, Cell membrane, Lipid-anchor, Multi-pass membrane protein, postsynaptic cell membrane, synapse |
| Calculated MW: | 60kDa/69kDa |
| Observed MW: | 70kDa |
| Synonyms: | CHRNA4, BFNC, EBN, EBN1, NACHR, NACHRA4, NACRA4 |
| Background: | This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | nAChRA4: a ligand-gated ion channels that binds acetylcholine and mediates fast signal transmission at synapses. After binding acetylcholine, these pentameric receptors undergo an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. An integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Membrane protein, multi-pass; Receptor, misc.; Channel, ligand-gated Chromosomal Location of Human Ortholog: 20q13.33 Cellular Component: cell soma; dendrite; external side of plasma membrane; membrane; nicotinic acetylcholine-gated receptor-channel complex; plasma membrane Molecular Function:acetylcholine binding; acetylcholine receptor activity; ligand-gated ion channel activity; nicotinic acetylcholine-activated cation-selective channel activity Biological Process: B cell activation; behavioral response to nicotine; calcium ion transport; cognition; DNA repair; membrane depolarization; neurological system process; neuromuscular synaptic transmission; regulation of action potential; regulation of dopamine secretion; regulation of inhibitory postsynaptic membrane potential; regulation of membrane potential; response to hypoxia; response to nicotine; response to oxidative stress; sensory perception of pain; signal transduction; synaptic transmission, cholinergic Disease: Epilepsy, Nocturnal Frontal Lobe, 1; Tobacco Addiction, Susceptibility To |
| NCBI Summary: | This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012] |
| UniProt Code: | P43681 |
| NCBI GenInfo Identifier: | 1351848 |
| NCBI Gene ID: | 1137 |
| NCBI Accession: | P43681.2 |
| UniProt Secondary Accession: | P43681,Q4JGR7, Q4VAQ5, Q4VAQ6, |
| UniProt Related Accession: | P43681 |
| Molecular Weight: | 60,994 Da |
| NCBI Full Name: | Neuronal acetylcholine receptor subunit alpha-4 |
| NCBI Synonym Full Names: | cholinergic receptor nicotinic alpha 4 subunit |
| NCBI Official Symbol: | CHRNA4 |
| NCBI Official Synonym Symbols: | EBN; BFNC; EBN1; NACHR; NACRA4; NACHRA4 |
| NCBI Protein Information: | neuronal acetylcholine receptor subunit alpha-4 |
| UniProt Protein Name: | Neuronal acetylcholine receptor subunit alpha-4 |
| Protein Family: | Neuronal acetylcholine receptor |
| UniProt Gene Name: | CHRNA4 |
| UniProt Entry Name: | ACHA4_HUMAN |
 | Western blot analysis of extracts of mouse kidney, using CHRNA4 antibody (CAB1648) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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