Signal Transduction Antibodies 2
Anti-CHRNA3 Antibody (CAB1674)
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- SKU:
- CAB1674
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-CHRNA3 Antibody |
| Antibody SKU: | CAB1674 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 32-240 of human CHRNA3 (NP_000734.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SH-SY5Y, HepG2, Mouse brain, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 32-240 of human CHRNA3 (NP_000734.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SEAE HRLF ERLF EDYN EIIR PVAN VSDP VIIH FEVS MSQL VKVD EVNQ IMET NLWL KQIW NDYK LKWN PSDY GGAE FMRV PAQK IWKP DIVL YNNA VGDF QVDD KTKA LLKY TGEV TWIP PAIF KSSC KIDV TYFP FDYQ NCTM KFGS WSYD KAKI DLVL IGSS MNLK DYWE SGEW AIIK APGY KHDI KYNC CEEI YPDI TYSL YIRR L |
| Gene ID: | 1136 |
| Uniprot: | P32297 |
| Cellular Location: | Cell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse |
| Calculated MW: | 55kDa/57kDa |
| Observed MW: | 57kDa |
| Synonyms: | CHRNA3, LNCR2, NACHRA3, PAOD2 |
| Background: | This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described. |
| UniProt Protein Function: | nAChRA3: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha- 3/CHRNA3 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, multi-pass; Membrane protein, integral; Channel, ligand-gated; Channel, cation Chromosomal Location of Human Ortholog: 15q24 Cellular Component: cell soma; dendrite; nicotinic acetylcholine-gated receptor-channel complex; plasma membrane; postsynaptic density Molecular Function:acetylcholine binding; acetylcholine receptor activity; ligand-gated ion channel activity; nicotinic acetylcholine-activated cation-selective channel activity Biological Process: behavioral response to nicotine; locomotory behavior; nervous system development; regulation of acetylcholine secretion; regulation of dendrite morphogenesis; regulation of excitatory postsynaptic membrane potential; regulation of membrane potential; regulation of smooth muscle contraction; signal transduction; synaptic transmission involved in micturition; synaptic transmission, cholinergic; transmembrane receptor protein tyrosine kinase activation (dimerization) Disease: Smoking As A Quantitative Trait Locus 3 |
| NCBI Summary: | This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009] |
| UniProt Code: | P32297 |
| NCBI GenInfo Identifier: | 254763435 |
| NCBI Gene ID: | 1136 |
| NCBI Accession: | P32297.4 |
| UniProt Secondary Accession: | P32297,Q15823, Q4KMN8, Q86U77, Q96RH3, Q99553, Q9BQ93 Q9BRR4, |
| UniProt Related Accession: | P32297 |
| Molecular Weight: | 55,637 Da |
| NCBI Full Name: | Neuronal acetylcholine receptor subunit alpha-3 |
| NCBI Synonym Full Names: | cholinergic receptor nicotinic alpha 3 subunit |
| NCBI Official Symbol: | CHRNA3 |
| NCBI Official Synonym Symbols: | LNCR2; PAOD2; NACHRA3 |
| NCBI Protein Information: | neuronal acetylcholine receptor subunit alpha-3 |
| UniProt Protein Name: | Neuronal acetylcholine receptor subunit alpha-3 |
| Protein Family: | Neuronal acetylcholine receptor |
| UniProt Gene Name: | CHRNA3 |
| UniProt Entry Name: | ACHA3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CHRNA3 antibody (CAB1674) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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