Name: Anti-CHRNA2 Antibody (CAB10056)
Brand: Signal Transduction Antibodies 1
SKU: CAB10056
Availability: OutOfStock

Antibody Name:	Anti-CHRNA2 Antibody
Antibody SKU:	CAB10056
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 27-264 of human CHRNA2 (NP_000733.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Rat
Positive Samples:	Rat skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 27-264 of human CHRNA2 (NP_000733.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EEAK RPPP RAPG DPLS SPSP TALP QGGS HTET EDRL FKHL FRGY NRWA RPVP NTSD VVIV RFGL SIAQ LIDV DEKN QMMT TNVW LKQE WSDY KLRW NPTD FGNI TSLR VPSE MIWI PDIV LYNN ADGE FAVT HMTK AHLF STGT VHWV PPAI YKSS CSID VTFF PFDQ QNCK MKFG SWTY DKAK IDLE QMEQ TVDL KDYW ESGE WAIV NATG TYNS KKYD CCAE IYPD VTYA FVIR RL
Gene ID:	1135
Uniprot:	Q15822
Cellular Location:	Cell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse
Calculated MW:	58kDa/59kDa
Observed MW:	60kDa

Synonyms:

CHRNA2

Background:

Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels formed by a pentameric arrangement of alpha and beta subunits to create distinct muscle and neuronal receptors. Neuronal receptors are found throughout the peripheral and central nervous system where they are involved in fast synaptic transmission. This gene encodes an alpha subunit that is widely expressed in the brain. The proposed structure for nAChR subunits is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. Mutations in this gene cause autosomal dominant nocturnal frontal lobe epilepsy type 4. Single nucleotide polymorphisms (SNPs) in this gene have been associated with nicotine dependence.

UniProt Protein Function:	nAChRA2: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Defects in CHRNA2 are the cause of nocturnal frontal lobe epilepsy type 4 (ENFL4). ENFL4 is an autosomal dominant epilepsy characterized by nocturnal seizures associated with fear sensation, tongue movements, and nocturnal wandering, closely resembling nightmares and sleep walking. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha- 2/CHRNA2 sub-subfamily.
UniProt Protein Details:	Protein type:Channel, cation; Channel, ligand-gated; Membrane protein, integral; Membrane protein, multi-pass; Receptor, misc. Chromosomal Location of Human Ortholog: 8p21 Cellular Component: nicotinic acetylcholine-gated receptor-channel complex; plasma membrane Molecular Function:acetylcholine binding; acetylcholine receptor activity; ligand-gated ion channel activity; nicotinic acetylcholine-activated cation-selective channel activity Biological Process: neuromuscular synaptic transmission; response to nicotine; signal transduction; synaptic transmission, cholinergic Disease: Epilepsy, Nocturnal Frontal Lobe, 4
NCBI Summary:	Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels formed by a pentameric arrangement of alpha and beta subunits to create distinct muscle and neuronal receptors. Neuronal receptors are found throughout the peripheral and central nervous system where they are involved in fast synaptic transmission. This gene encodes an alpha subunit that is widely expressed in the brain. The proposed structure for nAChR subunits is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. Mutations in this gene cause autosomal dominant nocturnal frontal lobe epilepsy type 4. Single nucleotide polymorphisms (SNPs) in this gene have been associated with nicotine dependence. [provided by RefSeq, Nov 2009]
UniProt Code:	Q15822
NCBI GenInfo Identifier:	308153405
NCBI Gene ID:	1135
NCBI Accession:	Q15822.2
UniProt Secondary Accession:	Q15822,Q9HAQ3, A8KAX3, B4DK19, J3KMY9,
UniProt Related Accession:	Q15822
Molecular Weight:	58,140 Da
NCBI Full Name:	Neuronal acetylcholine receptor subunit alpha-2
NCBI Synonym Full Names:	cholinergic receptor nicotinic alpha 2 subunit
NCBI Official Symbol:	CHRNA2
NCBI Protein Information:	neuronal acetylcholine receptor subunit alpha-2
UniProt Protein Name:	Neuronal acetylcholine receptor subunit alpha-2
Protein Family:	Neuronal acetylcholine receptor
UniProt Gene Name:	CHRNA2
UniProt Entry Name:	ACHA2_HUMAN

Western blot analysis of extracts of rat skeletal muscle, using CHRNA2 antibody (CAB10056) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

ELISA Kits

Human Neuronal acetylcholine receptor subunit alpha-2 (CHRNA2) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)