Antibody Name:	Anti-CHRM5 Antibody
Antibody SKU:	CAB5367
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 230-430 of human CHRM5 (NP_036257.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, U-251MG, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 230-430 of human CHRM5 (NP_036257.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LQGS DSVT KAEK RKPA HRAL FRSC LRCP RPTL AQRE RNQA SWSS SRRS TSTT GKPS QATG PSAN WAKA EQLT TCSS YPSS EDED KPAT DPVL QVVY KSQG KESP GEEF SAEE TEET FVKA ETEK SDYD TPNY LLSP AAAH RPKS QKCV AYKF RLVV KADG NQET NNGC HKVK IMPC PFPV AKEP STKG LNPN PSHQ MTKR K
Gene ID:	1133
Uniprot:	P08912
Cellular Location:	Cell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse
Calculated MW:	60kDa
Observed MW:	68kDa

Synonyms:

CHRM5, HM5

Background:

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, stimulation of this receptor is known to increase cyclic AMP levels.

UniProt Protein Function:	mAChR M5: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM5 sub-subfamily.
UniProt Protein Details:	Protein type:Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 15q26 Cellular Component: postsynaptic membrane; integral to plasma membrane; plasma membrane; cell junction Molecular Function:phosphoinositide phospholipase C activity; G-protein coupled acetylcholine receptor activity Biological Process: dopamine transport; gastric acid secretion; cell proliferation; acetylcholine receptor signaling, muscarinic pathway; metabolic process; muscarinic acetylcholine receptor, adenylate cyclase inhibiting pathway; transmission of nerve impulse
NCBI Summary:	The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, stimulation of this receptor is known to increase cyclic AMP levels. [provided by RefSeq, Jul 2008]
UniProt Code:	P08912
NCBI GenInfo Identifier:	543761
NCBI Gene ID:	1133
NCBI Accession:	P08912.2
UniProt Secondary Accession:	P08912,Q96RG7,
UniProt Related Accession:	P08912
Molecular Weight:	532
NCBI Full Name:	Muscarinic acetylcholine receptor M5
NCBI Synonym Full Names:	cholinergic receptor, muscarinic 5
NCBI Official Symbol:	CHRM5
NCBI Official Synonym Symbols:	HM5
NCBI Protein Information:	muscarinic acetylcholine receptor M5; acetylcholine receptor, muscarinic 5
UniProt Protein Name:	Muscarinic acetylcholine receptor M5
Protein Family:	Muscarinic acetylcholine receptor
UniProt Gene Name:	CHRM5
UniProt Entry Name:	ACM5_HUMAN

	Western blot analysis of extracts of various cell lines, using CHRM5 antibody (CAB5367) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 120s.
	Immunohistochemistry of paraffin-embedded rat brain using CHRM5 antibody (CAB5367) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using CHRM5 antibody (CAB5367). Blue: DAPI for nuclear staining.