Cell Biology Antibodies 6
Anti-CHRM2 Antibody (CAB1567)
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- SKU:
- CAB1567
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-CHRM2 Antibody |
| Antibody SKU: | CAB1567 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 208-388 of human CHRM2 (NP_001006629.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | SH-SY5Y, HepG2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 208-388 of human CHRM2 (NP_001006629.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | HISR ASKS RIKK DKKE PVAN QDPV SPSL VQGR IVKP NNNN MPSS DDGL EHNK IQNG KAPR DPVT ENCV QGEE KESS NDST SVSA VASN MRDD EITQ DENT VSTS LGHS KDEN SKQT CIRI GTKT PKSD SCTP TNTT VEVV GSSG QNGD EKQN IVAR KIVK MTKQ PAKK KPPP SREK KVTR T |
| Gene ID: | 1129 |
| Uniprot: | P08172 |
| Cellular Location: | Cell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse |
| Calculated MW: | 51kDa |
| Observed MW: | 52kDa |
| Synonyms: | CHRM2, HM2 |
| Background: | The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene. |
| UniProt Protein Function: | mAChR M2: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Genetic variations in CHRM2 can influence susceptibility to major depressive disorder (MDD). MDD is one of the most common psychiatric disorders. MDD is a complex trait characterized by one or more major depressive episodes without a history of manic, mixed, or hypomanic episodes. A major depressive episode is characterized by at least 2 weeks during which there is a new onset or clear worsening of either depressed mood or loss of interest or pleasure in nearly all activities. Four additional symptoms must also be present including changes in appetite, weight, sleep, and psychomotor activity; decreased energy; feelings of worthlessness or guilt; difficulty thinking, concentrating, or making decisions; or recurrent thoughts of death or suicidal ideation, plans, or attempts. The episode must be accompanied by distress or impairment in social, occupational, or other important areas of functioning. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily. |
| UniProt Protein Details: | Protein type:GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral Chromosomal Location of Human Ortholog: 7q31-q35 Cellular Component: postsynaptic membrane; integral to plasma membrane; plasma membrane; cell junction Molecular Function:G-protein coupled acetylcholine receptor activity Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein coupled receptor protein signaling pathway; nervous system development; G-protein signaling, coupled to cyclic nucleotide second messenger; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); acetylcholine receptor signaling, muscarinic pathway; response to virus; muscarinic acetylcholine receptor, phospholipase C activating pathway; regulation of heart contraction |
| NCBI Summary: | The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P08172 |
| NCBI GenInfo Identifier: | 113122 |
| NCBI Gene ID: | 1129 |
| NCBI Accession: | P08172.1 |
| UniProt Secondary Accession: | P08172,Q4VBK6, Q9P1X9, |
| UniProt Related Accession: | P08172 |
| Molecular Weight: | 466 |
| NCBI Full Name: | Muscarinic acetylcholine receptor M2 |
| NCBI Synonym Full Names: | cholinergic receptor, muscarinic 2 |
| NCBI Official Symbol: | CHRM2 |
| NCBI Official Synonym Symbols: | HM2 |
| NCBI Protein Information: | muscarinic acetylcholine receptor M2; 7TM receptor; muscarinic M2 receptor; acetylcholine receptor, muscarinic 2; cholinergic receptor, muscarinic 2, isoform a |
| UniProt Protein Name: | Muscarinic acetylcholine receptor M2 |
| Protein Family: | Muscarinic acetylcholine receptor |
| UniProt Gene Name: | CHRM2 |
| UniProt Entry Name: | ACM2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CHRM2 antibody (CAB1567) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of MCF-7 cells using CHRM2 antibody (CAB1567). Blue: DAPI for nuclear staining. |
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