KO Validated Antibodies 1

Anti-CHRAC1 Antibody (CAB14896)[KO Validated]

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SKU:
CAB14896
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CHRAC1 Antibody [KO Validated]
Antibody SKU:CAB14896
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-131 of human CHRAC1 (NP_059140.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-131 of human CHRAC1 (NP_059140.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MADV VVGK DKGG EQRL ISLP LSRI RVIM KSSP EVSS INQE ALVL TAKA TELF VQCL ATYS YRHG SGKE KKVL TYSD LANT AQQS ETFQ FLAD ILPK KILA SKYL KMLK EEKR EEDE ENDN DNES DHDE ADS
Gene ID:54108
Uniprot:Q9NRG0
Cellular Location:Nucleus
Calculated MW:14kDa
Observed MW:14kDa
Synonyms:CHRAC1, CHARC1, CHARC15, CHRAC-1, CHRAC-15, CHRAC15, YCL1
Background:
UniProt Protein Function:CHRAC1: Forms a complex with DNA polymerase epsilon subunit POLE3 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome remodeling activity of ISWI/SNF2H and ACF1.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: epsilon DNA polymerase complex; chromatin accessibility complex

Molecular Function:DNA binding; sequence-specific DNA binding; protein heterodimerization activity; DNA-directed DNA polymerase activity

Biological Process: chromatin remodeling

NCBI Summary:CHRAC1 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004]
UniProt Code:Q9NRG0
NCBI GenInfo Identifier:22653683
NCBI Gene ID:54108
NCBI Accession:Q9NRG0.1
UniProt Related Accession:Q9NRG0
Molecular Weight:14,711 Da
NCBI Full Name:Chromatin accessibility complex protein 1
NCBI Synonym Full Names:chromatin accessibility complex 1
NCBI Official Symbol:CHRAC1  
NCBI Official Synonym Symbols:YCL1; CHARC1; CHARC15; CHRAC-1; CHRAC15; CHRAC-15  
NCBI Protein Information:chromatin accessibility complex protein 1; DNA polymerase epsilon subunit p15; chromatin accessibility complex 15 kDa protein; histone-fold protein CHRAC15
UniProt Protein Name:Chromatin accessibility complex protein 1
UniProt Synonym Protein Names:Chromatin accessibility complex 15 kDa protein; CHRAC-15; HuCHRAC15; DNA polymerase epsilon subunit p15
Protein Family:Chromatin accessibility complex protein
UniProt Gene Name:CHRAC1  
UniProt Entry Name:CHRC1_HUMAN
Anti-CHRAC1 Antibody [KO Validated] (CAB14896)Western blot analysis of extracts from normal (control) and CHRAC1 knockout (KO) HeLa cells, using CHRAC1 antibody (CAB14896) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
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