Cell Biology Antibodies 6

Anti-CHP1 Antibody (CAB15791)

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SKU:
CAB15791
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CHP1 Antibody
Antibody SKU:CAB15791
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-195 of human CHP1 (NP_009167.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:LO2, 293T, HeLa, Mouse brain, Mouse heart, Mouse kidney, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-195 of human CHP1 (NP_009167.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGSR ASTL LRDE ELEE IKKE TGFS HSQI TRLY SRFT SLDK GENG TLSR EDFQ RIPE LAIN PLGD RIIN AFFP EGED QVNF RGFM RTLA HFRP IEDN EKSK DVNG PEPL NSRS NKLH FAFR LYDL DKDE KISR DELL QVLR MMVG VNIS DEQL GSIA DRTI QEAD QDGD SAIS FTEF VKVL EKVD VEQK MSIR FLH
Gene ID:11261
Uniprot:Q99653
Cellular Location:Cell membrane, Cytoplasm, Endoplasmic reticulum, Endoplasmic reticulum-Golgi intermediate compartment, Lipid-anchor, Membrane, Nucleus, cytoskeleton
Calculated MW:22kDa
Observed MW:20kDa
Synonyms:CHP1, CHP, SLC9A1BP, Sid470p, p22, p24
Background:This gene encodes a phosphoprotein that binds to the Na+/H+ exchanger NHE1. This protein serves as an essential cofactor which supports the physiological activity of NHE family members and may play a role in the mitogenic regulation of NHE1. The protein shares similarity with calcineurin B and calmodulin and it is also known to be an endogenous inhibitor of calcineurin activity.
UniProt Protein Function:Calcium-binding protein involved in different processes such as regulation of vesicular trafficking, plasma membrane Na+/H+ exchanger and gene transcription. Involved in the constitutive exocytic membrane traffic. Mediates the association between microtubules and membrane-bound organelles of the endoplasmic reticulum and Golgi apparatus and is also required for the targeting and fusion of transcytotic vesicles (TCV) with the plasma membrane. Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na+/H+ exchange activity. Affects the pH sensitivity of SLC9A1/NHE1 by increasing its sensitivity at acidic pH. Required for the stabilization and localization of SLC9A1/NHE1 at the plasma membrane. Inhibits serum- and GTPase-stimulated Na+/H+ exchange. Plays a role as an inhibitor of ribosomal RNA transcription by repressing the nucleolar UBF1 transcriptional activity. May sequester UBF1 in the nucleoplasm and limit its translocation to the nucleolus. Associates to the ribosomal gene promoter. Acts as a negative regulator of the calcineurin/NFAT signaling pathway. Inhibits NFAT nuclear translocation and transcriptional activity by suppressing the calcium-dependent calcineurin phosphatase activity. Also negatively regulates the kinase activity of the apoptosis-induced kinase STK17B. Inhibits both STK17B auto- and substrate-phosphorylations in a calcium-dependent manner.
NCBI Summary:This gene encodes a phosphoprotein that binds to the Na+/H+ exchanger NHE1. This protein serves as an essential cofactor which supports the physiological activity of NHE family members and may play a role in the mitogenic regulation of NHE1. The protein shares similarity with calcineurin B and calmodulin and it is also known to be an endogenous inhibitor of calcineurin activity. [provided by RefSeq, Jul 2008]
UniProt Code:Q99653
NCBI GenInfo Identifier:6005731
NCBI Gene ID:11261
NCBI Accession:NP_009167.1
UniProt Secondary Accession:Q99653,Q6FHZ9, B2R6H9,
UniProt Related Accession:Q99653
Molecular Weight:22,456 Da
NCBI Full Name:calcineurin B homologous protein 1
NCBI Synonym Full Names:calcineurin like EF-hand protein 1
NCBI Official Symbol:CHP1  
NCBI Official Synonym Symbols:CHP; p22; p24; Sid470p; SLC9A1BP  
NCBI Protein Information:calcineurin B homologous protein 1
UniProt Protein Name:Calcineurin B homologous protein 1
UniProt Synonym Protein Names:Calcineurin B-like protein; Calcium-binding protein CHP; Calcium-binding protein p22; EF-hand calcium-binding domain-containing protein p22
Protein Family:Chaoptin
UniProt Gene Name:CHP1  
Anti-CHP1 Antibody (CAB15791)Western blot analysis of extracts of various cell lines, using CHP1 antibody (CAB15791) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-CHP1 Antibody (CAB15791)Immunofluorescence analysis of L929 cells using CHP1 antibody (CAB15791) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-CHP1 Antibody (CAB15791)Immunofluorescence analysis of U-2 OS cells using CHP1 antibody (CAB15791) at dilution of 1:100. Blue: DAPI for nuclear staining.
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