Immunology Antibodies 1

Anti-CHMP2A Antibody (CAB10380)

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SKU:
CAB10380
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-CHMP2A Antibody
Antibody SKU:CAB10380
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-222 of human CHMP2A (NP_055268.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:A-431, HepG2, SW480
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-222 of human CHMP2A (NP_055268.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDLL FGRR KTPE ELLR QNQR ALNR AMRE LDRE RQKL ETQE KKII ADIK KMAK QGQM DAVR IMAK DLVR TRRY VRKF VLMR ANIQ AVSL KIQT LKSN NSMA QAMK GVTK AMGT MNRQ LKLP QIQK IMME FERQ AEIM DMKE EMMN DAID DAMG DEED EEES DAVV SQVL DELG LSLT DELS NLPS TGGS LSVA AGGK KAEA AASA LADA DADL EERL KNLR RD
Gene ID:27243
Uniprot:O43633
Cellular Location:Cytoplasmic side, Late endosome membrane, Peripheral membrane protein
Calculated MW:25kDa
Observed MW:37kDa
Synonyms:CHMP2A, BC-2, BC2, CHMP2, VPS2, VPS2A
Background:
UniProt Protein Function:CHMP2A: Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV- 1 p6- and p9-dependent virus release. Belongs to the SNF7 family.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 19q13.43

Cellular Component: cytosol; membrane; membrane coat; nuclear envelope

Molecular Function:phosphatidylcholine binding; protein binding; protein domain specific binding

Biological Process: cell separation during cytokinesis; endosome transport; establishment of protein localization; exit from mitosis; macroautophagy; membrane invagination; mitotic metaphase plate congression; nuclear envelope reassembly; nuclear organization and biogenesis; protein heterooligomerization; protein homooligomerization; protein polymerization; regulation of viral reproduction; viral infectious cycle

NCBI Summary:CHMP2A belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM, Mar 2008]
UniProt Code:O43633
NCBI GenInfo Identifier:73917740
NCBI Gene ID:27243
NCBI Accession:O43633.1
UniProt Secondary Accession:O43633,Q3ZTT0, B2R4W6,
UniProt Related Accession:O43633
Molecular Weight:25kDa
NCBI Full Name:Charged multivesicular body protein 2a
NCBI Synonym Full Names:charged multivesicular body protein 2A
NCBI Official Symbol:CHMP2A  
NCBI Official Synonym Symbols:BC2; BC-2; VPS2; CHMP2; VPS2A  
NCBI Protein Information:charged multivesicular body protein 2a
UniProt Protein Name:Charged multivesicular body protein 2a
UniProt Synonym Protein Names:Chromatin-modifying protein 2a; CHMP2a; Putative breast adenocarcinoma marker BC-2; Vacuolar protein sorting-associated protein 2-1; Vps2-1; hVps2-1
Protein Family:Charged multivesicular body protein
UniProt Gene Name:CHMP2A  
Anti-CHMP2A Antibody (CAB10380)Western blot analysis of extracts of various cell lines, using CHMP2A antibody (CAB10380) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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