Metabolism Antibodies 1
Anti-CHKA Antibody (CAB15040)
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- SKU:
- CAB15040
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-CHKA Antibody |
| Antibody SKU: | CAB15040 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human CHKA (NP_001268.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, Jurkat, Mouse brain, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human CHKA (NP_001268.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MKTK FCTG GEAE PSPL GLLL SCGS GSAA PAPG VGQQ RDAA SDLE SKQL GGQQ PPLA LPPP PPLP LPLP LPQP PPPQ PPAD EQPE PRTR RRAY LWCK EFLP GAWR GLRE DEFH ISVI RGGL SNML FQCS LPDT TATL GDEP RKVL LRLY GAIL QMRS CNKE GSEQ AQKE NE |
| Gene ID: | 1119 |
| Uniprot: | P35790 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 50kDa/52kDa |
| Observed MW: | 52kDa |
| Synonyms: | CHKA, CHK, CK, CKI, EK |
| Background: | The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. The protein encoded by this gene is the initial enzyme in the sequence and may play a regulatory role. The encoded protein also catalyzes the phosphorylation of ethanolamine. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | CHKA: Has a key role in phospholipid biosynthesis and may contribute to tumor cell growth. Catalyzes the first step in phosphatidylcholine biosynthesis. Contributes to phosphatidylethanolamine biosynthesis. Phosphorylates choline and ethanolamine. Has higher activity with choline. Belongs to the choline/ethanolamine kinase family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Lipid Metabolism - glycerophospholipid; EC 2.7.1.82; Kinase, other; EC 2.7.1.32 Chromosomal Location of Human Ortholog: 11q13.2 Cellular Component: cytosol Molecular Function:choline binding; signal transducer activity; protein homodimerization activity; cholinesterase activity; drug binding; ethanolamine kinase activity; choline kinase activity; ATP binding Biological Process: choline metabolic process; CDP-choline pathway; phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidylethanolamine biosynthetic process; phosphatidylcholine biosynthetic process; lipid metabolic process; signal transduction; lipid transport; phosphorylation |
| NCBI Summary: | The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. The protein encoded by this gene is the initial enzyme in the sequence and may play a regulatory role. The encoded protein also catalyzes the phosphorylation of ethanolamine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P35790 |
| NCBI GenInfo Identifier: | 226694197 |
| NCBI Gene ID: | 1119 |
| NCBI Accession: | P35790.3 |
| UniProt Secondary Accession: | P35790,Q8NE29, |
| UniProt Related Accession: | P35790 |
| Molecular Weight: | 457 |
| NCBI Full Name: | Choline kinase alpha |
| NCBI Synonym Full Names: | choline kinase alpha |
| NCBI Official Symbol: | CHKA |
| NCBI Official Synonym Symbols: | CK; EK; CHK; CKI |
| NCBI Protein Information: | choline kinase alpha; CHETK-alpha; ethanolamine kinase |
| UniProt Protein Name: | Choline kinase alpha |
| UniProt Synonym Protein Names: | CHETK-alpha; Ethanolamine kinase (EC:2.7.1.82); EK |
| UniProt Gene Name: | CHKA |
| UniProt Entry Name: | CHKA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CHKA antibody (CAB15040) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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